A3N1B3 (PLSB_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	APL_1107

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 824	824	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049426
Regions
	Motif	302 – 307	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A3N1B3 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 87574D88EC923FD2	FASTA	824	94,244
	10 20 30 40 50 60 MSSLLNFYRK VLNVPLSLLV KSRAIPTDPV KELNLNLEQP IIYVLPYTSQ TDLLILQKNC 70 80 90 100 110 120 LSLNLPDPLQ NNELNGQSLP RYVFLDEGRR FFKSKGAKSE TESIFYRYLD LHRNNESLDV 130 140 150 160 170 180 QLIPASVLWG RSPGKESEPH LRLMSSFQRI ISMIWFGRDN FVRFSQALSL KYMVAEHGAD 190 200 210 220 230 240 EGIAQKLARV AKIHFAKQRY SAMGPRLPDR QAMFNKIIQS PAIKVAIEEE AKTKKISIEK 250 260 270 280 290 300 ARQEAEKIVN EIAADVSHES LRIADRVLSW LWNKLYQGIN VQNGDRVRKL ALEGHEIVYV 310 320 330 340 350 360 PCHRSHMDYL LLSYLLYHQG LVPPHIAAGI NLNFFPAGPI FRSWGAFFIR RTFKGNRLYS 370 380 390 400 410 420 TIFREYLAEL FYRGYSVEYF IEGGRSRTGR LLEPKTGMMS MTLQALQRGL TRPISIVPVY 430 440 450 460 470 480 IGYEHVLEVD TYAKELRGAE KEKENAGLVL RVIKKLKNLG QCYVNFAEPI QVNNYLNQHF 490 500 510 520 530 540 PEWKESQAED SRPKWLNEAV DSVAHQVMIN INKAAAINAK NLIGSVLLAS RQRALAREQL 550 560 570 580 590 600 IEQVDSYLQL FKNVSYSDDA IVPNDNAEEM LNHVLTLPRS GVISEKDSFG EMIRLDRESA 610 620 630 640 650 660 VLMTYYRNNI QHLFVLPSLV ASIILHHESV SKDLIIKTVN RIYPFLKAEL FLHFEENDVR 670 680 690 700 710 720 NQVEAILTEF SAQRIVKYES DVLQINCVRV RALQLHAAGV REILQRYYIS LSILLEHPEI 730 740 750 760 770 780 SRAALEKESR SIAQRLSILH GINAPEFFDK ALFSTFSASL KAQGYFDSEG NCILEKAKEA 790 800 810 820 EEILRSLISV EVQLTIQGAM EKVEEVENTE TVVKTAEAVT EKNE

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN74199.1.
RefSeq	YP_001053804.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3N1B3.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N1B3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4849349.
GenomeReviews	Gene locus APL_1107 in contig CP000569_GR.
KEGG	apl:APL_1107.
PATRIC	20747126. VBIActPle94089_1155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	APLE416269:APL_1107-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_ACTP2

Accession

Primary (citable) accession number: A3N1B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program