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Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.UniRule annotation

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. Phosphatidate cytidylyltransferase (cdsA)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciAPLE416269:GHV7-1120-MONOMER.
UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferaseUniRule annotation (EC:2.3.1.15UniRule annotation)
Short name:
GPATUniRule annotation
Gene namesi
Name:plsBUniRule annotation
Ordered Locus Names:APL_1107
OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
Taxonomic identifieri416269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001432 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 824824Glycerol-3-phosphate acyltransferasePRO_1000049426Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi416269.APL_1107.

Structurei

3D structure databases

ProteinModelPortaliA3N1B3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi302 – 3076HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.UniRule annotation

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.

Sequencei

Sequence statusi: Complete.

A3N1B3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLLNFYRK VLNVPLSLLV KSRAIPTDPV KELNLNLEQP IIYVLPYTSQ
60 70 80 90 100
TDLLILQKNC LSLNLPDPLQ NNELNGQSLP RYVFLDEGRR FFKSKGAKSE
110 120 130 140 150
TESIFYRYLD LHRNNESLDV QLIPASVLWG RSPGKESEPH LRLMSSFQRI
160 170 180 190 200
ISMIWFGRDN FVRFSQALSL KYMVAEHGAD EGIAQKLARV AKIHFAKQRY
210 220 230 240 250
SAMGPRLPDR QAMFNKIIQS PAIKVAIEEE AKTKKISIEK ARQEAEKIVN
260 270 280 290 300
EIAADVSHES LRIADRVLSW LWNKLYQGIN VQNGDRVRKL ALEGHEIVYV
310 320 330 340 350
PCHRSHMDYL LLSYLLYHQG LVPPHIAAGI NLNFFPAGPI FRSWGAFFIR
360 370 380 390 400
RTFKGNRLYS TIFREYLAEL FYRGYSVEYF IEGGRSRTGR LLEPKTGMMS
410 420 430 440 450
MTLQALQRGL TRPISIVPVY IGYEHVLEVD TYAKELRGAE KEKENAGLVL
460 470 480 490 500
RVIKKLKNLG QCYVNFAEPI QVNNYLNQHF PEWKESQAED SRPKWLNEAV
510 520 530 540 550
DSVAHQVMIN INKAAAINAK NLIGSVLLAS RQRALAREQL IEQVDSYLQL
560 570 580 590 600
FKNVSYSDDA IVPNDNAEEM LNHVLTLPRS GVISEKDSFG EMIRLDRESA
610 620 630 640 650
VLMTYYRNNI QHLFVLPSLV ASIILHHESV SKDLIIKTVN RIYPFLKAEL
660 670 680 690 700
FLHFEENDVR NQVEAILTEF SAQRIVKYES DVLQINCVRV RALQLHAAGV
710 720 730 740 750
REILQRYYIS LSILLEHPEI SRAALEKESR SIAQRLSILH GINAPEFFDK
760 770 780 790 800
ALFSTFSASL KAQGYFDSEG NCILEKAKEA EEILRSLISV EVQLTIQGAM
810 820
EKVEEVENTE TVVKTAEAVT EKNE
Length:824
Mass (Da):94,244
Last modified:April 3, 2007 - v1
Checksum:i87574D88EC923FD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000569 Genomic DNA. Translation: ABN74199.1.
RefSeqiWP_009875486.1. NC_009053.1.

Genome annotation databases

EnsemblBacteriaiABN74199; ABN74199; APL_1107.
GeneIDi4849349.
KEGGiapl:APL_1107.
PATRICi20747126. VBIActPle94089_1155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000569 Genomic DNA. Translation: ABN74199.1.
RefSeqiWP_009875486.1. NC_009053.1.

3D structure databases

ProteinModelPortaliA3N1B3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416269.APL_1107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN74199; ABN74199; APL_1107.
GeneIDi4849349.
KEGGiapl:APL_1107.
PATRICi20747126. VBIActPle94089_1155.

Phylogenomic databases

eggNOGiENOG4105E55. Bacteria.
COG2937. LUCA.
HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.
OrthoDBiEOG6B625B.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.
BioCyciAPLE416269:GHV7-1120-MONOMER.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
    Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
    J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L20.

Entry informationi

Entry nameiPLSB_ACTP2
AccessioniPrimary (citable) accession number: A3N1B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: December 9, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.