A3N0S3 (SYC_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	APL_0913

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000332781
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N0S3 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 468A402A1AB03B62	FASTA	459	52,163
	10 20 30 40 50 60 MLKIYNTLKR EKEEFKPINP NQVGMYVCGV TVYDLCHFGH GRTFVSFDVI ARYLRYLGYN 70 80 90 100 110 120 LRYVRNITDV DDKIIKRALE NNETCDQLVD RMIAEMHKDF DDLNILRPDV EPRATKHIPE 130 140 150 160 170 180 IVAMVEKLIA NGHAYVAADG DVMFDVESFK KYGALSRQNL EQLQAGARVE IKSVKKNPMD 190 200 210 220 230 240 FVLWKMSKEG EPSWQSPWGN GRPGWHIECS AMNSKELGEH FDIHGGGSDL MFPHHENEIA 250 260 270 280 290 300 QSCCAHGGDY VNYWLHTGML TIDDEKMSKS LGNFFTIRTM LEKYESETLR YFFLTAHYRS 310 320 330 340 350 360 LLNYSLDNLD LARSALERLY TSLRGCDLSV EVAGGEQYVE AFKAAMDDDF NTPGALAVLF 370 380 390 400 410 420 EIAREVNKLK TEDMAKANGL AVRLKELAGV LGLLYQDPEA FLQGDADNDE VAEIEALIKQ 430 440 450 RNEAKAAKNW AVADEVRDKL KAMNIVLEDT PNGTTWRKA

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN74009.1.
RefSeq	YP_001053614.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3N0S3.
SMR	A3N0S3. Positions 1-458.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N0S3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4848686.
GenomeReviews	Gene locus APL_0913 in contig CP000569_GR.
KEGG	apl:APL_0913.
PATRIC	20746716. VBIActPle94089_0951.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	DFDALNM.
PhylomeDB	A3N0S3.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	APLE416269:APL_0913-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACTP2

Accession

Primary (citable) accession number: A3N0S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program