Reviewed, UniProtKB/Swiss-Prot A3N0K4 (QUEF_ACTP2)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	APL_0844

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000062326

Sequences

	Sequence		Length	Mass (Da)
	A3N0K4-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 98C808202965AE6D	FASTA	279	32,106
	10 20 30 40 50 60 MNYNDKSLSA LKLGQKTEYK SEYDPTLLQP VPRKLNRDGL GITEQQPFDR GADVWTCYEL 70 80 90 100 110 120 SWLNENGLPQ VAIADVAIDF RSENLIESKS FKLYLNSFNQ TKFASLEQVE QTLAKDLSQC 130 140 150 160 170 180 ASGQVSVKVY KLSAYTQQPI VDFAGECIDE QDIQIDSYEF SNEHLASVAE GEVVEETLVS 190 200 210 220 230 240 HLLKSNCLIT SQPDWGSVQI HYVGKKLNRE KLLRYLVSFR EHNEFHEQCV ERIFIDLIQF 250 260 270 TQPEKLTVYA RYTRRGGLDI NPFRSNFESV PQNLRMARQ

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN73940.1.
RefSeq	YP_001053545.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3N0K4.
Genome annotation databases
GeneID	4850362.
GenomeReviews	Gene locus APL_0844 in contig CP000569_GR.
KEGG	apl:APL_0844.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EHNEFHE.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_ACTP2

Accession

Primary (citable) accession number: A3N0K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)