Reviewed, UniProtKB/Swiss-Prot A3N0D6 (PYRD_ACTP2)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	APL_0774

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O(2) = orotate + H(2)O(2).
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Dihydroorotate dehydrogenase		PRO_1000024146
Sites
	Active site	171	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3N0D6-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 092E27DA1B4AFE3E	FASTA	335	36,192
	10 20 30 40 50 60 MYSLIRKCLF SMDAETAHNF SIQALKLAGK LPINVLPMPL NPVEVMGLQF KNPIGLAAGA 70 80 90 100 110 120 DKNGEAIDGF GKLGFGFIEV GTVTPVAQDG NPKPRQFRIL EAEGIINRNG FNNLGVDVLV 130 140 150 160 170 180 ENVKKAKYDG IIGINIGKNA VTPIERALDD YQICLRKVYE HADYITVNIS SPNTKNLRTL 190 200 210 220 230 240 QYGEALDDLL RSLKSEQESL SQKFNRYKPL VLKIAPDLTD EEIASVADSL VRYKIDGVIA 250 260 270 280 290 300 GNTTLSRDPV VGLKNAEQQG GLSGKPLNTL STRLISTLAK ELNGALPIIG SGGIHSVASG 310 320 330 QEKIDAGASL LQVYSAMIYQ GPALIQNLAK HIQVR

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN73872.1.
RefSeq	YP_001053477.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4849059.
GenomeReviews	Gene locus APL_0774 in contig CP000569_GR.
KEGG	apl:APL_0774.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. DHO_DHase_1_2. IPR005719. DHO_DHase_2. IPR001295. Dihydroorotate_DHase_core. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ACTP2

Accession

Primary (citable) accession number: A3N0D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 25, 2008
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)