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Protein

DNA topoisomerase 3

Gene

topB2

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141Magnesium 1; catalyticUniRule annotation
Metal bindingi110 – 1101Magnesium 1; catalyticUniRule annotation
Metal bindingi110 – 1101Magnesium 2UniRule annotation
Metal bindingi112 – 1121Magnesium 2UniRule annotation
Active sitei336 – 3361O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciAPLE416269:GHV7-754-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topB2Imported
Synonyms:topBUniRule annotation
Ordered Locus Names:APL_0744Imported
OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)Imported
Taxonomic identifieri416269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
Proteomesi
  • UP000001432 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei68 – 681Interaction with DNAUniRule annotation
Sitei177 – 1771Interaction with DNAUniRule annotation
Sitei185 – 1851Interaction with DNAUniRule annotation
Sitei192 – 1921Interaction with DNAUniRule annotation
Sitei338 – 3381Interaction with DNAUniRule annotation

Protein-protein interaction databases

STRINGi416269.APL_0744.

Structurei

3D structure databases

ProteinModelPortaliA3N0A8.
SMRiA3N0A8. Positions 8-616.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 141134ToprimInterPro annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni201 – 2066Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotationSAAS annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
HOGENOMiHOG000086848.
KOiK03169.
OMAiANQRYGF.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3N0A8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISEKRILRV FLAEKPSLAR AIADVLPKPH TRGDGYIQCG ENDTVTWCIG
60 70 80 90 100
HLLEQAEPDI YDERFKMWRM EHLPIVPQQW KLVPKKETLK QLKAVEKLVK
110 120 130 140 150
KADVLVNAGD PDREGQLLVD EVFSYLNVPP EKRNRIQRCL VSDLNPSAVK
160 170 180 190 200
KAIDKLQNNR DFIPLATSAL ARARADWLYG INMTRAYTLQ GRWAGYQGVL
210 220 230 240 250
SVGRVQTPVL GLIVRRDLEI ENFVPKDYFE VLAHIVVPET QERFTAQWQP
260 270 280 290 300
SKACEDYQDE DGRVLSKSLA ENVVKRITDQ AALVTDYQDK IETETAPLPY
310 320 330 340 350
SLSVLQIDAA RRYGLSAQAV LDICQKLYET HKLITYPRSD NRYLPNEHYA
360 370 380 390 400
DRFKVMAAIA HHLDEYKEKP EAVNPEIRNR CWNDKKVEAH HAIIPTVRQG
410 420 430 440 450
SVKLTENEKR IYQLIARQYL MQFCPDAEYR KGKIVLEIAG GTFNAQARNL
460 470 480 490 500
IVAGWKALLG KEDSDEVLEP LLPVVKKGQQ LHCEKGEIVS KKTQPPRHFT
510 520 530 540 550
DASLLSAMTG IARFVQDKQL KKVLRETDGL GTEATRAGII ELLFKRGFLI
560 570 580 590 600
KKGRNIHSTE AGRILISALP DEATQPDMTA HWEMQLTDIS KKQASYQQFM
610 620 630 640
QVLTSKLPEL LHTERQKLQN LSRIQPPASY RKRSNFKKNL QTAEK
Length:645
Mass (Da):73,724
Last modified:April 3, 2007 - v1
Checksum:i05253D352A652745
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000569 Genomic DNA. Translation: ABN73844.1.

Genome annotation databases

EnsemblBacteriaiABN73844; ABN73844; APL_0744.
KEGGiapl:APL_0744.
PATRICi20746358. VBIActPle94089_0779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000569 Genomic DNA. Translation: ABN73844.1.

3D structure databases

ProteinModelPortaliA3N0A8.
SMRiA3N0A8. Positions 8-616.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416269.APL_0744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN73844; ABN73844; APL_0744.
KEGGiapl:APL_0744.
PATRICi20746358. VBIActPle94089_0779.

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
HOGENOMiHOG000086848.
KOiK03169.
OMAiANQRYGF.
OrthoDBiEOG60CWM6.

Enzyme and pathway databases

BioCyciAPLE416269:GHV7-754-MONOMER.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
    Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.
    J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L20Imported.

Entry informationi

Entry nameiA3N0A8_ACTP2
AccessioniPrimary (citable) accession number: A3N0A8
Entry historyi
Integrated into UniProtKB/TrEMBL: April 3, 2007
Last sequence update: April 3, 2007
Last modified: July 6, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.