Reviewed, UniProtKB/Swiss-Prot A3N062 (ARGA_ACTP2)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	APL_0698

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity HAMAP MF_01105.
Miscellaneous	In bacteria which possess the bifunctional enzyme ornithine acetyltransferase/N-acetylglutamate synthase (argJ), argA fulfills an anaplerotic role. HAMAP MF_01105
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000084807
Regions
	Domain	289 – 429	141	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	A3N062-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: DDB00E770AB7F07F	FASTA	437	49,292
	10 20 30 40 50 60 MRNTELVQWF RQSTPYVNMH REKTFVIMLD GNAIAHPNFI NITNDIGLLH SLGIKLVIVF 70 80 90 100 110 120 GARCQIDELL AKNQMSSTYH KHIRITDSKT LEVVKQAVGG LHYDIFSRLS LRLPNSPVLN 130 140 150 160 170 180 VVSSNAVLAQ PLGVIDGVDY GLSGKIRRIN IEGIQQQLAQ DAIVVIGPIA PSVTGEMFNL 190 200 210 220 230 240 PFEEIATQIA IKLKADKLIG FCDQQGILDS EGNVLSDLHP REAKRYLTQF IESGQYHHSA 250 260 270 280 290 300 ARFLQAAIEA CHAGIKRSHL LSYKEDGSLL QELFSRDGIG TQLSEESSEN IRLATSFDIP 310 320 330 340 350 360 GLLNLIRPLE EQGILVKRSR EQLEMEISNY TIIERDGIVI ACAALNHYPQ EKMAEMACVA 370 380 390 400 410 420 VHPDYRDSSR GDVLLEAIKR RAYKLQVEKL FVLTTRTTQW FQERGFVLST TDDLPKEKRE 430 HYNYQRMSKI LILDLGQ

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN73798.1.
RefSeq	YP_001053403.1.
3D structure databases
SMR	A3N062. Positions 5-437.
ModBase	Search...
Protein-protein interaction databases
STRING	A3N062.
Genome annotation databases
GeneID	4848575.
GenomeReviews	Gene locus APL_0698 in contig CP000569_GR.
KEGG	apl:APL_0698.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG545264.
OMA	APYIHAF.
PhylomeDB	A3N062.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_ACTP2

Accession

Primary (citable) accession number: A3N062

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)