Reviewed, UniProtKB/Swiss-Prot A3N039 (SYN_ACTP2)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	APL_0675

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000051373

Sequences

	Sequence		Length	Mass (Da)
	A3N039-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 6EAEDBAEF1446C92	FASTA	467	52,727
	10 20 30 40 50 60 MSKFPTVSEI LSGKVAVGEE VAVRGWVRTR RDSKAGLSFL AVYDGSCFDP IQSIINNDLA 70 80 90 100 110 120 NYNDEVLRLT AGCSVIVTGK VVESPAEGQA VELHATHVEV VGWVEDPDTY PMAAKRHSIE 130 140 150 160 170 180 YLREVAHLRP RTNLIGAVAR VRHCLAQAIH RFFNEQGFYW VATPLITASD TEGAGEMFRV 190 200 210 220 230 240 STLDLENLPR TEEGKVDFSQ DFFGKESFLT VSGQLNGETY ACALSKVYTF GPTFRAENSN 250 260 270 280 290 300 TTRHLAEFWM VEPEFAFATL ADNAKLAEDM LKYVFKAVLE ERKDDMQFFA KHIDKDVITR 310 320 330 340 350 360 LENFIAAPFA QVDYTDAIEI LLKSGKEFEF PVSWGIDLSS EHERFLAEEY FKSPVVVKNY 370 380 390 400 410 420 PKDIKAFYMR LNDDGKTVAA MDVLAPGIGE IIGGSQREER LDVLDTRMVE MGLNPEDYWW 430 440 450 460 YRDLRKYGTV PHSGFGLGFE RLIVYVTGLQ NIREVIPFPR APRNANF

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000569 Genomic DNA. Translation: ABN73775.1.
RefSeq	YP_001053380.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3N039.
Genome annotation databases
GeneID	4850404.
GenomeReviews	Gene locus APL_0675 in contig CP000569_GR.
KEGG	apl:APL_0675.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ACTP2

Accession

Primary (citable) accession number: A3N039

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)