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A3MZU3

- PNP_ACTP2

UniProt

A3MZU3 - PNP_ACTP2

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, APL_0577
Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851Magnesium By similarity
Metal bindingi491 – 4911Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciAPLE416269:GHV7-586-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:APL_0577
OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
Taxonomic identifieri416269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001432: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 715715Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000329484Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi416269.APL_0577.

Structurei

3D structure databases

ProteinModelPortaliA3MZU3.
SMRiA3MZU3. Positions 616-690.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61160KHAdd
BLAST
Domaini621 – 68969S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3MZU3-1 [UniParc]FASTAAdd to Basket

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MNPIVKQFKY GQHTVTLETG AIARQATAAV MASMDDTTVF VTVVAKKEVK    50
EGQDFFPLTV DYQERTYAAG RIPGGFFKRE GRPSEGETLI ARLIDRPVRP 100
LFPEGFFNEI QVIATVVSVN PQISPDLVAM IGASAALSLS GVPFNGPIGA 150
ARVGFINDQF VLNPTTSEQK ISRLDLVVAG TDKAVLMVES EADILSEEQM 200
LSAVVFGHQQ QQVVIENIKE FVKEAGKPRW DWVAPEPNTA LINQVKALAE 250
ARIGDAYRIT EKQARYEQID AIKADVIAQL TAQDETVSEG AIIDIITALE 300
SSIVRGRIIA GEPRIDGRTV DTVRALDICT GVLPRTHGSA IFTRGETQAL 350
AVATLGTERD AQIIDELTGE KSDRFLFHYN FPPYSVGETG RIGSPKRREI 400
GHGRLAKRGV LAVMPTAEEF PYVVRVVSEI TESNGSSSMA SVCGASLALM 450
DAGVPIKAAV AGIAMGLVKE EEKFVVLSDI LGDEDHLGDM DFKVAGTREG 500
VTALQMDIKI EGITPEIMQI ALNQAKGARM HILSVMEQAI PAPRADISDF 550
APRIHTMKID PKKIKDVIGK GGAVIRALTE ETGTSIDIDD DGTVKIAATD 600
NNAAKAVMAR IEDIVAEVEV NAIYKGKVTR VVDFGAFVSI LGGKEGLVHI 650
SQITNERVER VADYLSVGQE VTVKVVEIDR QNRIRLTMKD LNNDTPVAEN 700
VTEEAEVSSE QQAEI 715
Length:715
Mass (Da):77,397
Last modified:April 3, 2007 - v1
Checksum:iEB37483A5031F627
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000569 Genomic DNA. Translation: ABN73679.1.
RefSeqiYP_001053284.1. NC_009053.1.

Genome annotation databases

EnsemblBacteriaiABN73679; ABN73679; APL_0577.
GeneIDi4849054.
KEGGiapl:APL_0577.
PATRICi20746012. VBIActPle94089_0608.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000569 Genomic DNA. Translation: ABN73679.1 .
RefSeqi YP_001053284.1. NC_009053.1.

3D structure databases

ProteinModelPortali A3MZU3.
SMRi A3MZU3. Positions 616-690.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416269.APL_0577.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN73679 ; ABN73679 ; APL_0577 .
GeneIDi 4849054.
KEGGi apl:APL_0577.
PATRICi 20746012. VBIActPle94089_0608.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci APLE416269:GHV7-586-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
    Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
    J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L20.

Entry informationi

Entry nameiPNP_ACTP2
AccessioniPrimary (citable) accession number: A3MZU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 3, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi