A3MZC5 (LPXA_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Short name=UDP-N-acetylglucosamine acyltransferase
EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	APL_0407

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus › Actinobacillus pleuropneumoniae › Actinobacillus pleuropneumoniae serovar 5

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine. HAMAP-Rule MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP-Rule MF_00387
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP-Rule MF_00387		PRO_0000302557

Sequences

	Sequence		Length	Mass (Da)
	A3MZC5 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 9BA73BCC47C1BDC5	FASTA	264	28,735
	10 20 30 40 50 60 MRLIDSTAKI SPLAVVEEGA QIGAHVEIGP FSVIGKNVKI GAKTVIHSHV VINGHTEIGE 70 80 90 100 110 120 QNQIFQFASI GEINQDLKYQ GEPTKVIIGD RNRIRENVTI HRGTVQGGGV TRIGNDNLFM 130 140 150 160 170 180 INTHIAHDCS IGNRCIIANN GTLAGHVTLD DFVIVGGMSA IHQFVVIGSH VMLGGGSMVS 190 200 210 220 230 240 QDVPPYVMAQ GNHAQPFGVN LEGLKRRGFD KPAMHAIRNA YKLIYRSGKT IEEAIPEIEQ 250 260 FAVNEPAVQL FLDFFKRSTR GIIR

References

[1]

"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN73511.1.
RefSeq	YP_001053116.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3MZC5.
SMR	A3MZC5. Positions 3-264.
ModBase	Search...
Protein-protein interaction databases
STRING	416269.APL_0407.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN73511; ABN73511; APL_0407.
GeneID	4850034.
KEGG	apl:APL_0407.
PATRIC	20745634. VBIActPle94089_0420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1043.
HOGENOM	HOG000294326.
KO	K00677.
OMA	REFCTFN.
ProtClustDB	PRK05289.
Enzyme and pathway databases
BioCyc	APLE416269:GHV7-486-MONOMER.
UniPathway	UPA00359; UER00477.
Family and domain databases
HAMAP	MF_00387. LpxA.
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR010137. Lipid_A_LpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
Pfam	PF00132. Hexapep. 4 hits. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01852. lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_ACTP2

Accession

Primary (citable) accession number: A3MZC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program