A3MZA3 (RISB_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	APL_0385

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000040359

Sequences

	Sequence		Length	Mass (Da)
	A3MZA3 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: BDD55D0AD5E3F840	FASTA	153	15,960
	10 20 30 40 50 60 MAKITGNLVA TGLKFGIVTA RFNDFINDKL LSGAIDTLVR HGAYENDIDT AWVPGAFEIP 70 80 90 100 110 120 LVAKKMATSG KYDAVICLGT VISGSTTHYD YVCNEAAKGI GAVALETGVP VIFGVLTTEN 130 140 150 IEQAIERAGT KAGNKGSECA LGAIEIVNVL KAI

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN73489.1.
RefSeq	YP_001053094.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3MZA3.
SMR	A3MZA3. Positions 1-149.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MZA3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4850446.
GenomeReviews	Gene locus APL_0385 in contig CP000569_GR.
KEGG	apl:APL_0385.
PATRIC	20745588. VBIActPle94089_0397.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0054.
HOGENOM	HBG311126.
OMA	DYVCNEA.
PhylomeDB	A3MZA3.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	APLE416269:APL_0385-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_ACTP2

Accession

Primary (citable) accession number: A3MZA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program