Reviewed, UniProtKB/Swiss-Prot A3MZ70 (SYM_ACTP2)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	APL_0352

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	679 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Homodimer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 679	679	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331773
Regions
	Domain	577 – 679	103	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	328 – 332	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	331	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MZ70-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: B8305A172717CE47	FASTA	679	77,118
	10 20 30 40 50 60 MSRKMLVTCA LPYANGAIHL GHMLEHIQAD IWVRFQRMRG NEIYFVCADD AHGTPIMLNA 70 80 90 100 110 120 AKQGITPEQL IEKAKADHVA DFKGFNISFD NYHSTHSEEN REITTEMYKK LRANGFIKSR 130 140 150 160 170 180 VISQLFDPEK QMFLPDRFVK GTCPKCKAED QYGDNCEVCA STYSPMDLIN PRSAISGATP 190 200 210 220 230 240 IVKESEHFFF DLPNFEGMLK EWTRSGSLQS EIANKMQEWF ESGLQQWDIS RDAPYFGFPI 250 260 270 280 290 300 PDAENKFFYV WLDAPIGYMA SFKNLCDRTG LNFDEFWKKD SETELYHFIG KDIVYFHSLF 310 320 330 340 350 360 WPAMLDGCEL RKPTNVFAHG YVTVDGVKMS KSRGTFIQAS TYLKHIDPEC LRYYYAAKLN 370 380 390 400 410 420 ERIEDLDLSL EDFVQRVNSD IVNKLVNLAS RNASFIAKRF EGKLADKLED EALFAEFIAQ 430 440 450 460 470 480 SEQIAAHYEN REFNKAIRLI MDLCDKANKY VDDKAPWVIA KQEGCDAQLQ AVCSMGIELF 490 500 510 520 530 540 RVLMSYLKPV LPQLAERAEA FLQTELTWDN IQQPLLGQNV APFKSLFSRL EKKQIDAVIE 550 560 570 580 590 600 ETKALFAAQN KEKGKQKVEN TENTAVEPIA AEITIDDFAK LDLRVAKVIS CEAVPESNKL 610 620 630 640 650 660 LKFQLDLGDH QRQVLSGIKA AYNNPEELVG RFVIMVANLA PRKMKFGVSE GMILSAGTGG 670 ADLFLLSADE GIRPGMQVK

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN73456.1. Different initiation.
RefSeq	YP_001053061.1.
3D structure databases
SMR	A3MZ70. Positions 2-543, 3-639, 573-678.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MZ70.
Genome annotation databases
GeneID	4849066.
GenomeReviews	Gene locus APL_0352 in contig CP000569_GR.
KEGG	apl:APL_0352.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HBG721667.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ACTP2

Accession

Primary (citable) accession number: A3MZ70

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	February 9, 2010
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)