A3MZ64 (GLGB_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	APL_0346

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 777	777	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_1000131804
Sites
	Active site	408	1	Nucleophile By similarity
	Active site	461	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MZ64 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 8420D9345EBD9E02	FASTA	777	89,300
	10 20 30 40 50 60 MKTYTYSKQD LSFIEQLSQA YCKDPFSYLG LHQAGDVSVI RVFLPEATTV KILSAVGQIL 70 80 90 100 110 120 SEALKIDDSG LFVAQLAQQY SSLNYRLRVG YSLAEIDLED PYRFTSSLLP MDNWLLAEGT 130 140 150 160 170 180 HLRPYEILGA HLKTQEGVSG VHFSVWAPNA RRVSVVGDFN YWDGRVNPMR FHAESGIWDI 190 200 210 220 230 240 FLPNVEKGAL YKFEILDSNG NIRLKSDPYA FASQFRPDTA SVVTGLPEKI EVDAKLRHAN 250 260 270 280 290 300 DPDQPISIYE VHLGSWRRHL ENNYWLNYEE IANELIPYVK DMGFTHIELL PITEYPFDGS 310 320 330 340 350 360 WGYQPTGLYS PTSRFGSPED LRTLIRKAHE AGINVILDWV VGHFPTDSHG LTEFDGSHLY 370 380 390 400 410 420 EHQDPREGYH QDWNTLIFNY GRHEVFNYLS SNALYWTERF GIDGLRVDAV SSMIYRDYSR 430 440 450 460 470 480 KDGEWIPNQY GGRENLEALD FLRRTNRMLK KEGHGAVVIA EESTSFAGIT HSPEENGVGF 490 500 510 520 530 540 DYKWNMGWMN DTLRYMSLDP IYRQYHHDWM TFGMMYQYSE KFVLPLSHDE VVHGKCSILG 550 560 570 580 590 600 KMSGDCWQKF ANLRAYYGYM WGYPGKKLLF MGNEFAQGRE WNYNESLDWF LLGEQGGGWH 610 620 630 640 650 660 KGVLNWVRDL NRTYQKYPAL YQLDYDPAGF EWLVVDDWQQ SVFAFERKAK NGESVIVVSN 670 680 690 700 710 720 FTPVVRHNYR IGVRQDGTYT EILNSDAAYY EGSNVGNYGE IECEAIESHG KPFSIELSIP 730 740 750 760 770 PLSTIFIACQ PKPKEAVEAE QDIVKMAEVA MQKALKPTKK TVSVKAKAHK KAHKNKK

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN73450.1.
RefSeq	YP_001053055.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3MZ64.
SMR	A3MZ64. Positions 120-727.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MZ64.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4849471.
GenomeReviews	Gene locus APL_0346 in contig CP000569_GR.
KEGG	apl:APL_0346.
PATRIC	20745504. VBIActPle94089_0355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	DGTCLYE.
PhylomeDB	A3MZ64.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	APLE416269:APL_0346-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_ACTP2

Accession

Primary (citable) accession number: A3MZ64

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program