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A3MZ64

- GLGB_ACTP2

UniProt

A3MZ64 - GLGB_ACTP2

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei408 – 4081NucleophileUniRule annotation
    Active sitei461 – 4611Proton donorUniRule annotation

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciAPLE416269:GHV7-355-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
    Alpha-(1->4)-glucan branching enzymeUniRule annotation
    Glycogen branching enzymeUniRule annotation
    Short name:
    BEUniRule annotation
    Gene namesi
    Name:glgBUniRule annotation
    Ordered Locus Names:APL_0346
    OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
    Taxonomic identifieri416269 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
    ProteomesiUP000001432: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7777771,4-alpha-glucan branching enzyme GlgBPRO_1000131804Add
    BLAST

    Proteomic databases

    PRIDEiA3MZ64.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi416269.APL_0346.

    Structurei

    3D structure databases

    ProteinModelPortaliA3MZ64.
    SMRiA3MZ64. Positions 120-727.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiGELSHAW.
    OrthoDBiEOG6JX7GT.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A3MZ64-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTYTYSKQD LSFIEQLSQA YCKDPFSYLG LHQAGDVSVI RVFLPEATTV    50
    KILSAVGQIL SEALKIDDSG LFVAQLAQQY SSLNYRLRVG YSLAEIDLED 100
    PYRFTSSLLP MDNWLLAEGT HLRPYEILGA HLKTQEGVSG VHFSVWAPNA 150
    RRVSVVGDFN YWDGRVNPMR FHAESGIWDI FLPNVEKGAL YKFEILDSNG 200
    NIRLKSDPYA FASQFRPDTA SVVTGLPEKI EVDAKLRHAN DPDQPISIYE 250
    VHLGSWRRHL ENNYWLNYEE IANELIPYVK DMGFTHIELL PITEYPFDGS 300
    WGYQPTGLYS PTSRFGSPED LRTLIRKAHE AGINVILDWV VGHFPTDSHG 350
    LTEFDGSHLY EHQDPREGYH QDWNTLIFNY GRHEVFNYLS SNALYWTERF 400
    GIDGLRVDAV SSMIYRDYSR KDGEWIPNQY GGRENLEALD FLRRTNRMLK 450
    KEGHGAVVIA EESTSFAGIT HSPEENGVGF DYKWNMGWMN DTLRYMSLDP 500
    IYRQYHHDWM TFGMMYQYSE KFVLPLSHDE VVHGKCSILG KMSGDCWQKF 550
    ANLRAYYGYM WGYPGKKLLF MGNEFAQGRE WNYNESLDWF LLGEQGGGWH 600
    KGVLNWVRDL NRTYQKYPAL YQLDYDPAGF EWLVVDDWQQ SVFAFERKAK 650
    NGESVIVVSN FTPVVRHNYR IGVRQDGTYT EILNSDAAYY EGSNVGNYGE 700
    IECEAIESHG KPFSIELSIP PLSTIFIACQ PKPKEAVEAE QDIVKMAEVA 750
    MQKALKPTKK TVSVKAKAHK KAHKNKK 777
    Length:777
    Mass (Da):89,300
    Last modified:April 3, 2007 - v1
    Checksum:i8420D9345EBD9E02
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000569 Genomic DNA. Translation: ABN73450.1.
    RefSeqiYP_001053055.1. NC_009053.1.

    Genome annotation databases

    EnsemblBacteriaiABN73450; ABN73450; APL_0346.
    GeneIDi4849471.
    KEGGiapl:APL_0346.
    PATRICi20745504. VBIActPle94089_0355.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000569 Genomic DNA. Translation: ABN73450.1 .
    RefSeqi YP_001053055.1. NC_009053.1.

    3D structure databases

    ProteinModelPortali A3MZ64.
    SMRi A3MZ64. Positions 120-727.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416269.APL_0346.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PRIDEi A3MZ64.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN73450 ; ABN73450 ; APL_0346 .
    GeneIDi 4849471.
    KEGGi apl:APL_0346.
    PATRICi 20745504. VBIActPle94089_0355.

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi GELSHAW.
    OrthoDBi EOG6JX7GT.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci APLE416269:GHV7-355-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
      Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
      J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: L20.

    Entry informationi

    Entry nameiGLGB_ACTP2
    AccessioniPrimary (citable) accession number: A3MZ64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3