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A3MZ64 (GLGB_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:APL_0346
OrganismActinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]
Taxonomic identifier416269 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length777 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7777771,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000131804

Sites

Active site4081Nucleophile By similarity
Active site4611Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A3MZ64 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 8420D9345EBD9E02

FASTA77789,300
        10         20         30         40         50         60 
MKTYTYSKQD LSFIEQLSQA YCKDPFSYLG LHQAGDVSVI RVFLPEATTV KILSAVGQIL 

        70         80         90        100        110        120 
SEALKIDDSG LFVAQLAQQY SSLNYRLRVG YSLAEIDLED PYRFTSSLLP MDNWLLAEGT 

       130        140        150        160        170        180 
HLRPYEILGA HLKTQEGVSG VHFSVWAPNA RRVSVVGDFN YWDGRVNPMR FHAESGIWDI 

       190        200        210        220        230        240 
FLPNVEKGAL YKFEILDSNG NIRLKSDPYA FASQFRPDTA SVVTGLPEKI EVDAKLRHAN 

       250        260        270        280        290        300 
DPDQPISIYE VHLGSWRRHL ENNYWLNYEE IANELIPYVK DMGFTHIELL PITEYPFDGS 

       310        320        330        340        350        360 
WGYQPTGLYS PTSRFGSPED LRTLIRKAHE AGINVILDWV VGHFPTDSHG LTEFDGSHLY 

       370        380        390        400        410        420 
EHQDPREGYH QDWNTLIFNY GRHEVFNYLS SNALYWTERF GIDGLRVDAV SSMIYRDYSR 

       430        440        450        460        470        480 
KDGEWIPNQY GGRENLEALD FLRRTNRMLK KEGHGAVVIA EESTSFAGIT HSPEENGVGF 

       490        500        510        520        530        540 
DYKWNMGWMN DTLRYMSLDP IYRQYHHDWM TFGMMYQYSE KFVLPLSHDE VVHGKCSILG 

       550        560        570        580        590        600 
KMSGDCWQKF ANLRAYYGYM WGYPGKKLLF MGNEFAQGRE WNYNESLDWF LLGEQGGGWH 

       610        620        630        640        650        660 
KGVLNWVRDL NRTYQKYPAL YQLDYDPAGF EWLVVDDWQQ SVFAFERKAK NGESVIVVSN 

       670        680        690        700        710        720 
FTPVVRHNYR IGVRQDGTYT EILNSDAAYY EGSNVGNYGE IECEAIESHG KPFSIELSIP 

       730        740        750        760        770 
PLSTIFIACQ PKPKEAVEAE QDIVKMAEVA MQKALKPTKK TVSVKAKAHK KAHKNKK 

« Hide

References

[1]"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: L20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000569 Genomic DNA. Translation: ABN73450.1.
RefSeqYP_001053055.1. NC_009053.1.

3D structure databases

ProteinModelPortalA3MZ64.
SMRA3MZ64. Positions 120-727.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416269.APL_0346.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEA3MZ64.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN73450; ABN73450; APL_0346.
GeneID4849471.
KEGGapl:APL_0346.
PATRIC20745504. VBIActPle94089_0355.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycAPLE416269:GHV7-355-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_ACTP2
AccessionPrimary (citable) accession number: A3MZ64
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 3, 2007
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries