A3MYX4 (ALR_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	APL_0252

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Alanine racemase HAMAP MF_01201		PRO_1000065967
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity
	Active site	271	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MYX4 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 856DA4B7839E89B1	FASTA	374	42,022
	10 20 30 40 50 60 MKPATATISR EALRHNIELI KTFAPQQKLL AMIKANAYGQ GLLPAADTLA DLVEGFGVAR 70 80 90 100 110 120 LREALEIQET GYTGKILLIE GFFDREELLK TLSRRFDTVI HCQEQLELLE QVAEEWQQEQ 130 140 150 160 170 180 QKGFWKRKAK IYFPINVWLK IDTGMHRLGV HPEQVDMFYQ RLKACPLVES ISFVSHFSRA 190 200 210 220 230 240 DEPDCGYTEK QIAIFEAATS PYPTHERSIS ASSGILYWKQ AHYDWVRPGI IMHGISPHYT 250 260 270 280 290 300 PITDLGFKPV MTLSSSLIAV RTHKAGEPVG YGGTWISDKD TKLGVVAMGY GDGYPRNAPE 310 320 330 340 350 360 GTPVLVNGRI VPIVGRVSMD MLTVDLGADS QDKVGDEVIF WGKDLLIEEV AQHIGVISYE 370 LITKLTPRVI FEYE

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN73360.1.
RefSeq	YP_001052965.1. NC_009053.1.
3D structure databases
ProteinModelPortal	A3MYX4.
SMR	A3MYX4. Positions 1-372.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MYX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4850055.
GenomeReviews	Gene locus APL_0252 in contig CP000569_GR.
KEGG	apl:APL_0252.
PATRIC	20745300. VBIActPle94089_0258.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	PTHERSI.
PhylomeDB	A3MYX4.
ProtClustDB	PRK00053.
Enzyme and pathway databases
BioCyc	APLE416269:APL_0252-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_ACTP2

Accession

Primary (citable) accession number: A3MYX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program