Reviewed, UniProtKB/Swiss-Prot A3MYW5 (ARGB_ACTP2)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	APL_0243

Organism

Actinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]

Taxonomic identifier

416269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable HAMAP MF_00082.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Acetylglutamate kinase HAMAP MF_00082		PRO_0000335605
Regions
	Region	66 – 67	2	Substrate binding By similarity
Sites
	Binding site	88	1	Substrate By similarity
	Binding site	179	1	Substrate By similarity
	Site	31	1	Transition state stabilizer By similarity
	Site	242	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MYW5-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: DA17538A33C71002	FASTA	284	30,318
	10 20 30 40 50 60 MLQNEVENFA NLLEQATVYL APYQEKIIVV KYGGNAMINE DRKKLVMQDI LLLNQLGVKV 70 80 90 100 110 120 VLVHGGGPEI SQGVKLLGKE PQFINGLRVT DQDTINVVLQ MLAGKVNKSL VALLKGKGVG 130 140 150 160 170 180 LCGIDANMLQ CEKLQAEVDY GFVGEIVKVN TQLLELALSA NLIPVISTVG VDDQGVAYNI 190 200 210 220 230 240 NADTVASEIA MALGAAKLVS MTDIAGLLRD RFDESTLIPE VEVSEVQGLI DQGIIAGGMI 250 260 270 280 PKIACCTDFI NAGGIEANII DGRVPHAILV SLFGGKNGTL FYKK

References

[1]	"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)." Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E. J. Bacteriol. 190:1495-1496(2008) [PubMed: 18065534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000569 Genomic DNA. Translation: ABN73351.1.
RefSeq	YP_001052956.1.
3D structure databases
SMR	A3MYW5. Positions 7-283.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MYW5.
Genome annotation databases
GeneID	4850320.
GenomeReviews	Gene locus APL_0243 in contig CP000569_GR.
KEGG	apl:APL_0243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	MDIVEMV.
PhylomeDB	A3MYW5.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. IPR001057. Glu_5kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_ACTP2

Accession

Primary (citable) accession number: A3MYW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)