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A3MYL1

- LEUC_ACTP2

UniProt

A3MYL1 - LEUC_ACTP2

Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

    Catalytic activityi

    (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi408 – 4081Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi411 – 4111Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciAPLE416269:GHV7-144-MONOMER.
    UniPathwayiUPA00048; UER00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
    Alternative name(s):
    Alpha-IPM isomeraseUniRule annotation
    Short name:
    IPMIUniRule annotation
    Isopropylmalate isomeraseUniRule annotation
    Gene namesi
    Name:leuCUniRule annotation
    Ordered Locus Names:APL_0139
    OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
    Taxonomic identifieri416269 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
    ProteomesiUP000001432: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4694693-isopropylmalate dehydratase large subunitPRO_1000063519Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of LeuC and LeuD.UniRule annotation

    Protein-protein interaction databases

    STRINGi416269.APL_0139.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0065.
    HOGENOMiHOG000226972.
    KOiK01703.
    OMAiQARAKTM.
    OrthoDBiEOG600DP5.

    Family and domain databases

    Gene3Di3.30.499.10. 3 hits.
    3.40.1060.10. 1 hit.
    HAMAPiMF_01026. LeuC_type1.
    InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR00170. leuC. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3MYL1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKTLYEKLF DAHVVYEAAG ETPILYINRH LIHEVTSPQA FDGLRVAGRQ    50
    VRQIGKTFGT MDHSISTQVR DVNKLEGQAK IQVLELAKNC EANGISLFDM 100
    QTKEQGIVHV MGPEQGLTLP GMTIVCGDSH TATHGAFGAL AFGIGTSEVE 150
    HVLATQTLKQ ARAKSMKVEV RGKVNPGITA KDIVLAIIGK TTMAGGTGHV 200
    VEFCGEAIRN LSMEGRMTVC NMAIEFGAKA GLVAPDETTF AYLKDRPHAP 250
    KGKDWDDAVE YWTTLKSDDD AVFDSVVVLE AKDIAPQVTW GTNPGQVIGI 300
    DQVVPNPQEM ADPVTKASAE KALAYIGLDA NTDMKNIPVD QVFIGSCTNS 350
    RIEDLRAAAA VMKGRKKADN VKRVLVVPGS GLVKEQAEKE GLDKIFIEAG 400
    AEWRNPGCSM CLGMNDDRLG EWERCASTSN RNFEGRQGRN GRTHLVSPAM 450
    AAAAAMFGKF VDIRHVELN 469
    Length:469
    Mass (Da):50,705
    Last modified:April 3, 2007 - v1
    Checksum:iF6DC751553AD0E6B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000569 Genomic DNA. Translation: ABN73247.1.
    RefSeqiYP_001052852.1. NC_009053.1.

    Genome annotation databases

    EnsemblBacteriaiABN73247; ABN73247; APL_0139.
    GeneIDi4848700.
    KEGGiapl:APL_0139.
    PATRICi20745068. VBIActPle94089_0143.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000569 Genomic DNA. Translation: ABN73247.1 .
    RefSeqi YP_001052852.1. NC_009053.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416269.APL_0139.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN73247 ; ABN73247 ; APL_0139 .
    GeneIDi 4848700.
    KEGGi apl:APL_0139.
    PATRICi 20745068. VBIActPle94089_0143.

    Phylogenomic databases

    eggNOGi COG0065.
    HOGENOMi HOG000226972.
    KOi K01703.
    OMAi QARAKTM.
    OrthoDBi EOG600DP5.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00071 .
    BioCyci APLE416269:GHV7-144-MONOMER.

    Family and domain databases

    Gene3Di 3.30.499.10. 3 hits.
    3.40.1060.10. 1 hit.
    HAMAPi MF_01026. LeuC_type1.
    InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR00170. leuC. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
      Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
      J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: L20.

    Entry informationi

    Entry nameiLEUC_ACTP2
    AccessioniPrimary (citable) accession number: A3MYL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3