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A3MYL1

- LEUC_ACTP2

UniProt

A3MYL1 - LEUC_ACTP2

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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi408 – 4081Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi411 – 4111Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciAPLE416269:GHV7-144-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:APL_0139
OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
Taxonomic identifieri416269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001432: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4694693-isopropylmalate dehydratase large subunitPRO_1000063519Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi416269.APL_0139.

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiQARAKTM.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3MYL1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKTLYEKLF DAHVVYEAAG ETPILYINRH LIHEVTSPQA FDGLRVAGRQ
60 70 80 90 100
VRQIGKTFGT MDHSISTQVR DVNKLEGQAK IQVLELAKNC EANGISLFDM
110 120 130 140 150
QTKEQGIVHV MGPEQGLTLP GMTIVCGDSH TATHGAFGAL AFGIGTSEVE
160 170 180 190 200
HVLATQTLKQ ARAKSMKVEV RGKVNPGITA KDIVLAIIGK TTMAGGTGHV
210 220 230 240 250
VEFCGEAIRN LSMEGRMTVC NMAIEFGAKA GLVAPDETTF AYLKDRPHAP
260 270 280 290 300
KGKDWDDAVE YWTTLKSDDD AVFDSVVVLE AKDIAPQVTW GTNPGQVIGI
310 320 330 340 350
DQVVPNPQEM ADPVTKASAE KALAYIGLDA NTDMKNIPVD QVFIGSCTNS
360 370 380 390 400
RIEDLRAAAA VMKGRKKADN VKRVLVVPGS GLVKEQAEKE GLDKIFIEAG
410 420 430 440 450
AEWRNPGCSM CLGMNDDRLG EWERCASTSN RNFEGRQGRN GRTHLVSPAM
460
AAAAAMFGKF VDIRHVELN
Length:469
Mass (Da):50,705
Last modified:April 3, 2007 - v1
Checksum:iF6DC751553AD0E6B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000569 Genomic DNA. Translation: ABN73247.1.
RefSeqiYP_001052852.1. NC_009053.1.

Genome annotation databases

EnsemblBacteriaiABN73247; ABN73247; APL_0139.
GeneIDi4848700.
KEGGiapl:APL_0139.
PATRICi20745068. VBIActPle94089_0143.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000569 Genomic DNA. Translation: ABN73247.1 .
RefSeqi YP_001052852.1. NC_009053.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416269.APL_0139.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN73247 ; ABN73247 ; APL_0139 .
GeneIDi 4848700.
KEGGi apl:APL_0139.
PATRICi 20745068. VBIActPle94089_0143.

Phylogenomic databases

eggNOGi COG0065.
HOGENOMi HOG000226972.
KOi K01703.
OMAi QARAKTM.
OrthoDBi EOG600DP5.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci APLE416269:GHV7-144-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
    Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
    J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L20.

Entry informationi

Entry nameiLEUC_ACTP2
AccessioniPrimary (citable) accession number: A3MYL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: April 3, 2007
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3