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A3MYB6

- SYI_ACTP2

UniProt

A3MYB6 - SYI_ACTP2

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (03 Apr 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei562 – 5621Aminoacyl-adenylateUniRule annotation
    Binding sitei606 – 6061ATPUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciAPLE416269:GHV7-44-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:APL_0044
    OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
    Taxonomic identifieri416269 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
    ProteomesiUP000001432: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_1000022036Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi416269.APL_0044.

    Structurei

    3D structure databases

    ProteinModelPortaliA3MYB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi603 – 6075"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3MYB6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDYKNTLNL PETGFPMRGD LAKREPDMLK NWYDKNLYQK VRESSKGKKS    50
    FILHDGPPYA NGNIHIGHAV NKILKDIIMK SKTALGFDTP YVPGWDCHGL 100
    PIELKVEGIV GKPNEKISAA EFRQACRDYA KEQVEGQKAD FIRMGILGDW 150
    DNPYLTMNFD TEAHIIRTLG KVIANGHLYK GSKPVHWCLD CGSSLAEAEV 200
    EYEDKVSPSI YVRFKAIDSA AVEAKFNAVG KGSGQISAVI WTTTPWTLPS 250
    NKAISINPEF DYQLVQFGDE RVVLVKDLVE SVQKAVGVES VEVLGEVKGD 300
    ALELMQFQHP FYDYSVPLIL GDHVTTDGGT GLVHTAPDHG QDDFVVSKKY 350
    NIEMAGLVAN DGKFISSTPF FAGLGVFESN EKVLEKLKEV GALLKLERIK 400
    HSYPHCWRHK TPIIFRATPQ WFIGMETQGL REQALGEIKS VRWIPSWGEA 450
    RIDTMVANRP DWCISRQRTW GVPMTMFVHN ETEELHPRTL EILESVAKRV 500
    EEKGIQAWWD LDPVEVLGEE DAKNYRKVPD TLDVWFDSGS TYASVVEARP 550
    EFNGNSTDMY LEGSDQHRGW FMSSLMLSTA TNGKAPYKQV LTHGFVVDEK 600
    GRKMSKSLGN VIVPSEVWNK NGADILRLWV ASTDYTGEIA VSHNILNSAG 650
    ESYRRIRNTA RFLLANLNGF DPKRDLVKPE EMIALDRWAV SCALEAQNDI 700
    KEAYDNYQFH TVVQRLMRFC SIEMGSFYLD IIKDRQYTTK ADSLARRSCQ 750
    TALWHIAEAL VRWMAPILSF TADEIWSYLP QVEGRSEFVF TEEFYEGLFG 800
    LTEADKLDDA YWQQILKVRA ESNRVLEQAR KDKVIGSGLE AKVTLYANNE 850
    IRAMLEQLGN ELRFVLITSQ AIIKPLSEAD VAEGEMAGLA VKVERAEGEK 900
    CPRCWHFATD IGTHTEHSSV CGRCVENVAG EGEKRSFA 938
    Length:938
    Mass (Da):105,762
    Last modified:April 3, 2007 - v1
    Checksum:i0525D82650508888
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000569 Genomic DNA. Translation: ABN73152.1.
    RefSeqiYP_001052757.1. NC_009053.1.

    Genome annotation databases

    EnsemblBacteriaiABN73152; ABN73152; APL_0044.
    GeneIDi4849600.
    KEGGiapl:APL_0044.
    PATRICi20744864. VBIActPle94089_0046.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000569 Genomic DNA. Translation: ABN73152.1 .
    RefSeqi YP_001052757.1. NC_009053.1.

    3D structure databases

    ProteinModelPortali A3MYB6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416269.APL_0044.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN73152 ; ABN73152 ; APL_0044 .
    GeneIDi 4849600.
    KEGGi apl:APL_0044.
    PATRICi 20744864. VBIActPle94089_0046.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci APLE416269:GHV7-44-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
      Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
      J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: L20.

    Entry informationi

    Entry nameiSYI_ACTP2
    AccessioniPrimary (citable) accession number: A3MYB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: April 3, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3