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A3MYB6

- SYI_ACTP2

UniProt

A3MYB6 - SYI_ACTP2

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Protein

Isoleucine--tRNA ligase

Gene
ileS, APL_0044
Organism
Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei562 – 5621Aminoacyl-adenylate By similarity
Binding sitei606 – 6061ATP By similarity
Metal bindingi901 – 9011Zinc By similarity
Metal bindingi904 – 9041Zinc By similarity
Metal bindingi921 – 9211Zinc By similarity
Metal bindingi924 – 9241Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciAPLE416269:GHV7-44-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:APL_0044
OrganismiActinobacillus pleuropneumoniae serotype 5b (strain L20)
Taxonomic identifieri416269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus
ProteomesiUP000001432: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Isoleucine--tRNA ligaseUniRule annotationPRO_1000022036Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi416269.APL_0044.

Structurei

3D structure databases

ProteinModelPortaliA3MYB6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi603 – 6075"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3MYB6-1 [UniParc]FASTAAdd to Basket

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MIDYKNTLNL PETGFPMRGD LAKREPDMLK NWYDKNLYQK VRESSKGKKS    50
FILHDGPPYA NGNIHIGHAV NKILKDIIMK SKTALGFDTP YVPGWDCHGL 100
PIELKVEGIV GKPNEKISAA EFRQACRDYA KEQVEGQKAD FIRMGILGDW 150
DNPYLTMNFD TEAHIIRTLG KVIANGHLYK GSKPVHWCLD CGSSLAEAEV 200
EYEDKVSPSI YVRFKAIDSA AVEAKFNAVG KGSGQISAVI WTTTPWTLPS 250
NKAISINPEF DYQLVQFGDE RVVLVKDLVE SVQKAVGVES VEVLGEVKGD 300
ALELMQFQHP FYDYSVPLIL GDHVTTDGGT GLVHTAPDHG QDDFVVSKKY 350
NIEMAGLVAN DGKFISSTPF FAGLGVFESN EKVLEKLKEV GALLKLERIK 400
HSYPHCWRHK TPIIFRATPQ WFIGMETQGL REQALGEIKS VRWIPSWGEA 450
RIDTMVANRP DWCISRQRTW GVPMTMFVHN ETEELHPRTL EILESVAKRV 500
EEKGIQAWWD LDPVEVLGEE DAKNYRKVPD TLDVWFDSGS TYASVVEARP 550
EFNGNSTDMY LEGSDQHRGW FMSSLMLSTA TNGKAPYKQV LTHGFVVDEK 600
GRKMSKSLGN VIVPSEVWNK NGADILRLWV ASTDYTGEIA VSHNILNSAG 650
ESYRRIRNTA RFLLANLNGF DPKRDLVKPE EMIALDRWAV SCALEAQNDI 700
KEAYDNYQFH TVVQRLMRFC SIEMGSFYLD IIKDRQYTTK ADSLARRSCQ 750
TALWHIAEAL VRWMAPILSF TADEIWSYLP QVEGRSEFVF TEEFYEGLFG 800
LTEADKLDDA YWQQILKVRA ESNRVLEQAR KDKVIGSGLE AKVTLYANNE 850
IRAMLEQLGN ELRFVLITSQ AIIKPLSEAD VAEGEMAGLA VKVERAEGEK 900
CPRCWHFATD IGTHTEHSSV CGRCVENVAG EGEKRSFA 938
Length:938
Mass (Da):105,762
Last modified:April 3, 2007 - v1
Checksum:i0525D82650508888
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000569 Genomic DNA. Translation: ABN73152.1.
RefSeqiYP_001052757.1. NC_009053.1.

Genome annotation databases

EnsemblBacteriaiABN73152; ABN73152; APL_0044.
GeneIDi4849600.
KEGGiapl:APL_0044.
PATRICi20744864. VBIActPle94089_0046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000569 Genomic DNA. Translation: ABN73152.1 .
RefSeqi YP_001052757.1. NC_009053.1.

3D structure databases

ProteinModelPortali A3MYB6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416269.APL_0044.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN73152 ; ABN73152 ; APL_0044 .
GeneIDi 4849600.
KEGGi apl:APL_0044.
PATRICi 20744864. VBIActPle94089_0046.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci APLE416269:GHV7-44-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
    Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
    J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L20.

Entry informationi

Entry nameiSYI_ACTP2
AccessioniPrimary (citable) accession number: A3MYB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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