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A3MY92 (DDL_ACTP2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:APL_0020
OrganismActinobacillus pleuropneumoniae serotype 5b (strain L20) [Complete proteome] [HAMAP]
Taxonomic identifier416269 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030421

Regions

Domain104 – 300197ATP-grasp
Nucleotide binding132 – 18756ATP By similarity

Sites

Metal binding2541Magnesium or manganese 1 By similarity
Metal binding2671Magnesium or manganese 1 By similarity
Metal binding2671Magnesium or manganese 2 By similarity
Metal binding2691Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3MY92 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 620151A5C69B3D2E

FASTA30332,704
        10         20         30         40         50         60 
MSIKDEKIAV LFGGVSQERE VSLNSGAAVT EALKSLGYNV EGIDTKDFPI EKLKEKGIQR 

        70         80         90        100        110        120 
VFNILHGGIG ENGVLQGALE QMGIPYTGCG VMASAVTLDK FRTKLMWQAV GLPTADMVVV 

       130        140        150        160        170        180 
RRGEAVDSEQ IIAKLGLPVF VKPSSEGSSV GVTKVKTVEQ LLPAVEEALK FDSIVLVEAF 

       190        200        210        220        230        240 
LAGKEYSVPV LDGQVLPAVQ IIPEGEFYDY HAKYISDNTQ YLVPALSDDR QAEVAELVKA 

       250        260        270        280        290        300 
AYEVVGCRGW SRIDVMEDAN GHFNLVEVNT CPGMTSHSIF PKSAATVGIP FEKLVERVLE 


LSA 

« Hide

References

[1]"The complete genome sequence of Actinobacillus pleuropneumoniae L20 (serotype 5b)."
Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M., Nash J.H.E.
J. Bacteriol. 190:1495-1496(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: L20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000569 Genomic DNA. Translation: ABN73128.1.
RefSeqYP_001052733.1. NC_009053.1.

3D structure databases

ProteinModelPortalA3MY92.
SMRA3MY92. Positions 6-302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416269.APL_0020.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN73128; ABN73128; APL_0020.
GeneID4849242.
KEGGapl:APL_0020.
PATRIC20744816. VBIActPle94089_0022.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycAPLE416269:GHV7-20-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ACTP2
AccessionPrimary (citable) accession number: A3MY92
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways