A3MXQ7RTCA_PYRCJRNA 3'-terminal phosphate cyclaseRNA cyclaseRNA-3'-phosphate cyclase6.5.1.4rtcAPcal_2009Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)ArchaeaThermoproteotaThermoproteiThermoprotealesThermoproteaceaePyrobaculumComplete sequence of Pyrobaculum calidifontis JCM 11548.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphateCytoplasmBelongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.ATP-bindingCytoplasmLigaseNucleotide-bindingATPATPMIRIDGSYGEGGGQILRTAIALSALLGVPVEVFNIRAKRANPGLQPQHLTGVNAAALLTDAELEGAAKGSTRLVFKPKTIKCGGFQIDIGTAGSISLVIQTLTPIALFAPCPVRLSITGGTDVAWAPPIDYMRFVFAPVVGRFGGRVKIEVLRRGHYPKGGGRVEVEVAPVDVLKPVDAVDFGKVLKIRGISHAVNLPAHVAERQARAAAEALAKEGYAADISTEVRNDGLGPGSGVVLWAESDVGNVVGGDALGERGKPAEAVGREAAEKLLTALKSGASVDSHMADMAVVYMALAEGVSRITTPELTLHLQTNMYIVERFLPVKFRVEKLGQRHLIEVNGVGRRR
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