A3MXP1 (SYA_PYRCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Pcal_1993

Organism

Pyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]

Taxonomic identifier

410359 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	896 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 896	896	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_1000074514
Sites
	Metal binding	599	1	Zinc By similarity
	Metal binding	603	1	Zinc By similarity
	Metal binding	707	1	Zinc By similarity
	Metal binding	711	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MXP1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: D44383DF1B4BA50E	FASTA	896	100,700
	10 20 30 40 50 60 MQKTQMLSAE IFRRRGHIRK QCPLCKSHFW TLRPDQEVCG DQPCTPYGFI GNPPTKVAVE 70 80 90 100 110 120 SLRDVRERFL SFFERHGHAR IKRYPVVARW RDDVYLVGAS IYDFQPWVTS GAVPPPANPL 130 140 150 160 170 180 TISQPSIRLT DVDKVGRSGR HLTGFEMMAH HAFNYPDKYV YWIDETTEYA YRFFTEELGI 190 200 210 220 230 240 PPEEITFKES MWEGGGNAGE CFEVLVRGLE AATLVFMHYE VKDGKYVELP LKIVDTGYGL 250 260 270 280 290 300 ERIYWLIKGT PTIYDAVFGP YLAKVRERLG VPEPPRDVMA KASIYFGQMD PEVISLDKAY 310 320 330 340 350 360 DIIAEKIGVD GKWLREVVKP QEALYVLADH SRTVAWMIAD GVIPSNSGAG YLARLLIRRA 370 380 390 400 410 420 LRGLMLAGVD APLVELFDLH LKELRYDYPE VWEARSLILE LVDMEEKKYR EVLKSAPAVV 430 440 450 460 470 480 KRALEENRRR GKAGLDKEDL VTLYDSYGLP PEVVAEAAKS MGIEVKVPDD FYSLLASRHV 490 500 510 520 530 540 RREKGREATL VEMAKVADLP RTRELFYEDA YMRTFKAKVL RVIDGKYVVL DQTAFYAEGG 550 560 570 580 590 600 GQPADTGVLR HSGGVAKVVD VQRVGHVIVH VVEGDVPQEG SEVVGEVDWE RRYALMKMHT 610 620 630 640 650 660 GTHVLIQSIR RVLGPHIWQA GAQKDIPFSR IDVTHYKLPT PEEVAKIERL ANDVVQRDLP 670 680 690 700 710 720 VYVKVMPRNE AEAKYGFILY QGGVVPAREI RVVQIGPDDE PYDVQACGGT HLRRTGEIGL 730 740 750 760 770 780 IKVHKVERIA DGVVRFIFTT GPHAVAYVQE LERQAAEAAA LGGGSKEELV EVVKRLLKRA 790 800 810 820 830 840 EEAEKKARHY AELYAAALAE NLKAEAVGQR RLAVVELDDE ELAKRVALLA TKRDPDLVLV 850 860 870 880 890 VKAGERVTIY TGGVDVGPIV KALREIGFRG GGSKTFAQGV YAGDVEKLKE AIRRAL

References

[1]

"Complete sequence of Pyrobaculum calidifontis JCM 11548." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 11548 / VA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000561 Genomic DNA. Translation: ABO09408.1.
RefSeq	YP_001056874.1. NC_009073.1.
3D structure databases
ProteinModelPortal	A3MXP1.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MXP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4908986.
GenomeReviews	Gene locus Pcal_1993 in contig CP000561_GR.
KEGG	pcl:Pcal_1993.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	A3MXP1.
ProtClustDB	PRK13902.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_PYRCJ

Accession

Primary (citable) accession number: A3MXP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program