A3MXD4 (PURL_PYRCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Pcal_1885

Organism

Pyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]

Taxonomic identifier

410359 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050338
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A3MXD4 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 33BFFCBCD8269E44	FASTA	693	73,651
	10 20 30 40 50 60 MALTAAELEA IRRGLGRGPT PVELALFTAH WSEHCAYKST RGRLSRLPSK APWVVKGPGT 70 80 90 100 110 120 DAPLVEVAPG LYVTFKIESH NHPSAVDPYN GAATGVGGII RDILTVGAKP IALLVNLHFG 130 140 150 160 170 180 PPQDAHAKWI AQGVVRGISD YGNRVGVPVV GGETWFDEDF TYTPIVLATC VGVVSAEDVP 190 200 210 220 230 240 RGGVEAGDLF IVVGSGADKS GLGGSAFASK TLTEEEDLGA VQVADPLMGK RLIDLVQEAR 250 260 270 280 290 300 QCVKYIKDLG GGGLATAAAE LAHWFGLGLE IDLDKIHMSD AEMGPAEVLI SETQERLVLV 310 320 330 340 350 360 TSEGQLPCLK ALFEKYDVPY SVVGRFTEGG RLVFKWRGEV VGDVPVELAA NAPVLEWPTR 370 380 390 400 410 420 PYKAKPLPDL PQPPLDKAID AVLSSPNVAK KAAIYERFDF DVGVRTVVKP GEGDAAVLKL 430 440 450 460 470 480 LELGDVGIVV KGDANPRYTY LSPRLGAANA FVKAYRNVVV ARGIPLAAVD SINLGSPARP 490 500 510 520 530 540 EVYWQFVEAV EGLAEAAEAL GVPIVGGKVS LYNEYLDKPI KPVVAVVVLG KIDDVGKAAK 550 560 570 580 590 600 ATWEAGDGIY LWGVTKAEVG GSEYLYRIFG EVAGEPPAVD YAVEKEILRL VGTWRPRKAT 610 620 630 640 650 660 DVGLGGLAAA LAKMAVNSGV GADVDICKAP AETTRLDFLL FSESNGRFIT AGEEGPGVKI 670 680 690 GEAEGEKLRL RCGGTLLYKR KVEELRELMA LRL

References

[1]

"Complete sequence of Pyrobaculum calidifontis JCM 11548." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 11548 / VA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000561 Genomic DNA. Translation: ABO09301.1.
RefSeq	YP_001056767.1. NC_009073.1.
3D structure databases
ProteinModelPortal	A3MXD4.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MXD4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4908391.
GenomeReviews	Gene locus Pcal_1885 in contig CP000561_GR.
KEGG	pcl:Pcal_1885.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PYRCJ

Accession

Primary (citable) accession number: A3MXD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program