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A3MX72 (SYW_PYRCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:Pcal_1822
OrganismPyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]
Taxonomic identifier410359 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_1000018997

Regions

Motif81 – 899"HIGH" region HAMAP-Rule MF_00140
Motif258 – 2625"KMSKS" region HAMAP-Rule MF_00140

Sequences

Sequence LengthMass (Da)Tools
A3MX72 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: 23BE96FD004632BA

FASTA37442,675
        10         20         30         40         50         60 
MDEEFVVTPW EVRGRVDYQK LMQQFGARPL TQAEVSLLEK YAGEVHPLIR RGFFYAHRDL 

        70         80         90        100        110        120 
DAILKWHGEG KPWALYTGRG PSGPVHIGHM VPWILLKWLS DKFGVEVYFQ MTDDEKFYDD 

       130        140        150        160        170        180 
PEMRLEEATK WAYENALDVI ALGFTPDKLH LIIDTKDIKP LYPIAAKVAK KLTWNTVKAT 

       190        200        210        220        230        240 
FGFTDSTNIG LIFYPSLQIA VAFLPTELHG RPTPVLIPCA IDQDPYFRLA RDIAESLGYP 

       250        260        270        280        290        300 
KPATLYSKFI MALTGESKMS ASNPNSAIYT IDDEKTIRRK IANAYTGGRP TAEEQRRLGG 

       310        320        330        340        350        360 
NPDVCPVYHY HMLFDPDDAS VEKIYHSCKS GALLCGECKQ MLYEKIRKFV KQHQEAREKA 

       370 
RDKVDAYRLS SKLS 

« Hide

References

[1]"Complete sequence of Pyrobaculum calidifontis JCM 11548."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 11548 / VA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000561 Genomic DNA. Translation: ABO09239.1.
RefSeqYP_001056705.1. NC_009073.1.

3D structure databases

ProteinModelPortalA3MX72.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING410359.Pcal_1822.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO09239; ABO09239; Pcal_1822.
GeneID4909500.
KEGGpcl:Pcal_1822.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000224742.
KOK01867.
OMAKPETAIY.
ProtClustDBPRK12285.

Enzyme and pathway databases

BioCycPCAL410359:GIX2-1865-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_A. Trp_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_PYRCJ
AccessionPrimary (citable) accession number: A3MX72
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries