A3MWV9 (A3MWV9_PYRCJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase RuleBase RU000515
EC=4.2.1.24 RuleBase RU000515

Gene names

Ordered Locus Names:

Pcal_1709

Organism

Pyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]

Taxonomic identifier

410359 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O. RuleBase RU000515
Subunit structure	Homooctamer By similarity. PIRSR PIRSR001415-3 RuleBase RU000515
Sequence similarities	Belongs to the ALADH family. RuleBase RU004161

Ontologies

Keywords
Biological process	Porphyrin biosynthesis RuleBase RU000515
Ligand	Magnesium PIRSR PIRSR001415-5 Metal-binding PIRSR PIRSR001415-3 Zinc PIRSR PIRSR001415-3
Molecular function	Lyase RuleBase RU000515 EMBL ABO09126.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	204	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Active site	257	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Metal binding	125	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	127	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	135	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	242	1	Magnesium By similarity PIRSR PIRSR001415-5
	Binding site	214	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	226	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	284	1	Substrate 2 By similarity PIRSR PIRSR001415-2
	Binding site	323	1	Substrate 2 By similarity PIRSR PIRSR001415-2

Sequences

	Sequence		Length	Mass (Da)
	A3MWV9 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 1C517E1477286B43	FASTA	338	37,676
	10 20 30 40 50 60 MRVQFPTTRP RRLRASKIIR DAVAETQIDA GDFIYPLFVK PGGEREPIGP MPGIYRWPVG 70 80 90 100 110 120 RELINHVEEA LSLGINKFIL FGVLPDELKN PEGTGGYDPE GVVPRAIRLI KEIFGDRVLV 130 140 150 160 170 180 FADVCLCEYT DHGHCGVVKE KRDRWYVDND ETIKLYAKEA VVYAEAGADF VAPSGMMDGQ 190 200 210 220 230 240 VREIRRALDA HGFEEVGIMA YSAKYASAFY GPFRVAAASA PKFGDRRTYQ MDPRNAYEAL 250 260 270 280 290 300 KEVAMDLEEG ADIVMVKPAL AYLDVIRLVK QHFPWVPLAA YNVSGEYSLV KAAATAGYVD 310 320 330 ERTITLEILT AIKRAGADLI LTYHALEAAK WIKEGLPF

References

[1]

"Complete sequence of Pyrobaculum calidifontis JCM 11548." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 11548 / VA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000561 Genomic DNA. Translation: ABO09126.1.
RefSeq	YP_001056592.1. NC_009073.1.
3D structure databases
ProteinModelPortal	A3MWV9.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MWV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4908900.
GenomeReviews	Gene locus Pcal_1709 in contig CP000561_GR.
KEGG	pcl:Pcal_1709.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05029.
HOGENOM	HBG285270.
OMA	TEAYNEN.
PhylomeDB	A3MWV9.
ProtClustDB	PRK09283.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01698.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A3MWV9_PYRCJ

Accession

Primary (citable) accession number: A3MWV9

Entry history

Integrated into UniProtKB/TrEMBL:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)