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Protein

Malate dehydrogenase

Gene

mdh

Organism
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311NADUniRule annotation
Binding sitei80 – 801SubstrateUniRule annotation
Binding sitei86 – 861SubstrateUniRule annotation
Binding sitei93 – 931NADUniRule annotation
Binding sitei118 – 1181SubstrateUniRule annotation
Binding sitei149 – 1491SubstrateUniRule annotation
Active sitei173 – 1731Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 116NADUniRule annotation
Nucleotide bindingi116 – 1183NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPCAL410359:GIX2-1735-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Pcal_1699
OrganismiPyrobaculum calidifontis (strain JCM 11548 / VA1)
Taxonomic identifieri410359 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
ProteomesiUP000001431: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Malate dehydrogenasePRO_1000026483Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi410359.Pcal_1699.

Structurei

3D structure databases

ProteinModelPortaliA3MWU9.
SMRiA3MWU9. Positions 1-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiAGNVGEH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

A3MWU9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITIVGSGRV GTAAAAIMGI MRIDKKILLI DIVKGLPQGE ALDLNHMSAI
60 70 80 90 100
LGLDVEYEGS NDYKDMAGSD LVIVTAGFPR KPGMTREQLV ETNAKIVSDI
110 120 130 140 150
GKEIKKYAPD SVVILTTNPL DAMTYVMWKS TGFPRERVIG FSGVLDAGRL
160 170 180 190 200
AYYAAKKLGI SPASILPIVL GQHGESMFPV PSKSFVHGVP LSKLLSEDQL
210 220 230 240 250
REVVEETVKA GAKITELRGF SSNWGPGAGL AIMADSVKRD ARRSLIASVV
260 270 280 290 300
LKGEYGVFDL PVEVPIVLGK TGVVKVLEIE LTPEEKEKFN QSVEAIRKLV

GTIPQSYLQ
Length:309
Mass (Da):33,293
Last modified:April 3, 2007 - v1
Checksum:i04CB46D8FD7EEBCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000561 Genomic DNA. Translation: ABO09116.1.
RefSeqiYP_001056582.1. NC_009073.1.

Genome annotation databases

EnsemblBacteriaiABO09116; ABO09116; Pcal_1699.
GeneIDi4909393.
KEGGipcl:Pcal_1699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000561 Genomic DNA. Translation: ABO09116.1.
RefSeqiYP_001056582.1. NC_009073.1.

3D structure databases

ProteinModelPortaliA3MWU9.
SMRiA3MWU9. Positions 1-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi410359.Pcal_1699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO09116; ABO09116; Pcal_1699.
GeneIDi4909393.
KEGGipcl:Pcal_1699.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiAGNVGEH.

Enzyme and pathway databases

BioCyciPCAL410359:GIX2-1735-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 11548 / VA1.

Entry informationi

Entry nameiMDH_PYRCJ
AccessioniPrimary (citable) accession number: A3MWU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: January 7, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.