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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NAD; via amide nitrogenUniRule annotation1
Binding sitei60NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei124NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi154Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi169IronUniRule annotation1
Binding sitei219NAD; via amide nitrogenUniRule annotation1
Binding sitei229GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 53NADUniRule annotation2
Nucleotide bindingi152 – 154NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Pcal_1555
OrganismiPyrobaculum calidifontis (strain JCM 11548 / VA1)
Taxonomic identifieri410359 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000001431 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001156141 – 261Thiamine thiazole synthaseAdd BLAST261

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi410359.Pcal_1555.

Structurei

3D structure databases

ProteinModelPortaliA3MWF6.
SMRiA3MWF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG0949018Y.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

A3MWF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKIGRAII KHGLEDLYEY SDVDVAIVGA GPAGLTAARY LAERGFKVLV
60 70 80 90 100
FERRFSFGGG IGPGGNMIPK IVVQEEALPI LKDFKVRFKP AGDGLYTVDP
110 120 130 140 150
AELIAKLAAG AIDAGAKILL GVHVDDVIFR GDPPRVAGLL WIWTPIQMSG
160 170 180 190 200
MHVDPLYTQA KAVIDATGHD AEVVSVAARK VPELGLQLQG EKSAWSEVSE
210 220 230 240 250
KLVVEHTGRV APGLYVAGIA VCAVYGLPRM GPIFGGMLMS GRKVAEVVAK
260
DLAEVAYAVR A
Length:261
Mass (Da):27,712
Last modified:April 3, 2007 - v1
Checksum:i625AA2032525819E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000561 Genomic DNA. Translation: ABO08973.1.

Genome annotation databases

EnsemblBacteriaiABO08973; ABO08973; Pcal_1555.
KEGGipcl:Pcal_1555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000561 Genomic DNA. Translation: ABO08973.1.

3D structure databases

ProteinModelPortaliA3MWF6.
SMRiA3MWF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi410359.Pcal_1555.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO08973; ABO08973; Pcal_1555.
KEGGipcl:Pcal_1555.

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG0949018Y.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_PYRCJ
AccessioniPrimary (citable) accession number: A3MWF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 3, 2007
Last modified: February 15, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.