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A3MW09

- LIPA_PYRCJ

UniProt

A3MW09 - LIPA_PYRCJ

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Protein
Probable lipoyl synthase
Gene
lipA, Pcal_1406
Organism
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi33 – 331Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi38 – 381Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi44 – 441Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi59 – 591Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi63 – 631Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi66 – 661Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPCAL410359:GIX2-1438-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Pcal_1406
OrganismiPyrobaculum calidifontis (strain JCM 11548 / VA1)
Taxonomic identifieri410359 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
ProteomesiUP000001431: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Probable lipoyl synthaseUniRule annotation
PRO_0000325324Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi410359.Pcal_1406.

Structurei

3D structure databases

ProteinModelPortaliA3MW09.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiTIRAVRH.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A3MW09-1 [UniParc]FASTAAdd to Basket

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MELPSWIRVK AGDYGRIVAV REAVFAAGVH TICEEAHCPN IFSCWGEGTA    50
TFLILGDVCT RACKFCAVKT GDPRGFVDPT EPARVAEAVA KLGLRYVVIT 100
SVDRDDLPDG GASQFASVVK AVKARAPWAK VEVLTPDFGG SAEAVASVVE 150
AGPDVYAHNL ETVRRLTPLV RDRRASYDVS LRVLKMAKEL GAVTKSGLMV 200
GLGETFDEVL EALSDLRRVD VDIVTIGQYL KPRGHKRFLE VQRWVPPEEF 250
EKYREAAEAM GFKAVVAGPL VRSSYKAHEA YLEMLRKTIG R 291
Length:291
Mass (Da):31,867
Last modified:April 3, 2007 - v1
Checksum:i18F94A58A1299FBE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000561 Genomic DNA. Translation: ABO08826.1.
RefSeqiYP_001056292.1. NC_009073.1.

Genome annotation databases

EnsemblBacteriaiABO08826; ABO08826; Pcal_1406.
GeneIDi4909119.
KEGGipcl:Pcal_1406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000561 Genomic DNA. Translation: ABO08826.1 .
RefSeqi YP_001056292.1. NC_009073.1.

3D structure databases

ProteinModelPortali A3MW09.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 410359.Pcal_1406.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO08826 ; ABO08826 ; Pcal_1406 .
GeneIDi 4909119.
KEGGi pcl:Pcal_1406.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi TIRAVRH.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci PCAL410359:GIX2-1438-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 11548 / VA1.

Entry informationi

Entry nameiLIPA_PYRCJ
AccessioniPrimary (citable) accession number: A3MW09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: April 3, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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