A3MV29 (RIFK_PYRCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	Pcal_1071

Organism

Pyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]

Taxonomic identifier

410359 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Riboflavin kinase HAMAP MF_01285		PRO_0000322101
Regions
	Nucleotide binding	93 – 98	6	CDP By similarity
	Nucleotide binding	192 – 195	4	CDP By similarity
	Region	1 – 83	83	Unknown HAMAP MF_01285
	Region	84 – 212	129	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	122	1	Magnesium By similarity
	Metal binding	124	1	Magnesium By similarity
	Binding site	179	1	FMN By similarity
	Binding site	187	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MV29 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 55FB8E799B8F28F1	FASTA	212	23,505
	10 20 30 40 50 60 MTELYCERKT LADLIAFASV EGLPVGEAAK RLCMSRQGAY KAVKALREAG YLSEGPVIKL 70 80 90 100 110 120 TQKGRDALSI VLRNLLRYFD IASIKLVGRV VTGLGEGAFY MSLEGYRRAI ERELGFTPYP 130 140 150 160 170 180 GTLNIKLEPQ SLAHRRYLDG LPGIHIPGFT NGMRTYGAVK AFRARLADVE GAVVMPERTH 190 200 210 HPVDVIEFIA PVRVRDVLGL KDGDRVELEV YL

References

[1]

"Complete sequence of Pyrobaculum calidifontis JCM 11548." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 11548 / VA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000561 Genomic DNA. Translation: ABO08496.1.
RefSeq	YP_001055962.1. NC_009073.1.
3D structure databases
ProteinModelPortal	A3MV29.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MV29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4909736.
GenomeReviews	Gene locus Pcal_1071 in contig CP000561_GR.
KEGG	pcl:Pcal_1071.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG10832.
HOGENOM	HBG553349.
OMA	MPERTHH.
ProtClustDB	CLSK631895.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023470. Riboflavin_kinase_archaeal. IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_PYRCJ

Accession

Primary (citable) accession number: A3MV29

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	April 3, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program