A3MUA2 (A3MUA2_PYRCJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193 RuleBase RU003812
EC=6.3.1.5 HAMAP MF_00193 RuleBase RU003812

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Pcal_0793

Organism

Pyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]

Taxonomic identifier

410359 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 RuleBase RU003812
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 RuleBase RU004252
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193 RuleBase RU003811

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 RuleBase RU003811 NAD HAMAP MF_00193 RuleBase RU003811 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 RuleBase RU003811 EMBL ABO08219.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	48 – 55	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	50	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A3MUA2 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 95393B53B10C0742	FASTA	276	30,321
	10 20 30 40 50 60 MFKKVSCFLS VDPKSVVDAV DYDKAASIIV EFIVRQVGEA GARGVVVGLS GGIDSTVAAA 70 80 90 100 110 120 LAVKALGSER VLGLIMPSVF TPVEDVRDAE EVAERLGIRM RKVDITPIVE SFKRSIPDYV 130 140 150 160 170 180 EDRLASGNLL PRIRMTILYY YANRDNLLVL GTGDRSELLL GYFTKYGDGG VDLLPIGGLY 190 200 210 220 230 240 KLQVREMARR LGFSKIAAKP SSPRLWPGHM AEAELGATYD VIDPILFAVF DMGKPVDEVK 250 260 270 RVYGPVVDLV LQRVQRNRHK LNPPPIPDLA PARRNV

References

[1]

"Complete sequence of Pyrobaculum calidifontis JCM 11548." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 11548 / VA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000561 Genomic DNA. Translation: ABO08219.1.
RefSeq	YP_001055685.1. NC_009073.1.
3D structure databases
ProteinModelPortal	A3MUA2.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MUA2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4909470.
GenomeReviews	Gene locus Pcal_0793 in contig CP000561_GR.
KEGG	pcl:Pcal_0793.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	DQTDADE.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A3MUA2_PYRCJ

Accession

Primary (citable) accession number: A3MUA2

Entry history

Integrated into UniProtKB/TrEMBL:	April 3, 2007
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)