A3MU00 (SYL_PYRCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	Pcal_0691

Organism

Pyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]

Taxonomic identifier

410359 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum › Pyrobaculum calidifontis

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	Leucine--tRNA ligase HAMAP-Rule MF_00049		PRO_1000009404
Regions
	Motif	43 – 53	11	"HIGH" region HAMAP-Rule MF_00049
	Motif	638 – 642	5	"KMSKS" region HAMAP-Rule MF_00049
Sites
	Binding site	641	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MU00 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: F0D3BC3EC9CB671B	FASTA	946	107,481
	10 20 30 40 50 60 MSELAKFFIE VAEKWQRRWA EAKVFEPSPQ PGRPKFFITA AYPYPNGTIH IGHGRTYLVA 70 80 90 100 110 120 DVMARFRRHL GYNVLFPMAF HYTGTPILTI AEVIAAGDKA VIEEYKEIYG VSDDDIKKMG 130 140 150 160 170 180 DPLYLAQYFH RRSKEAMIKF GLGIDWSREF TTIDPEYQRF IQWQFEKLRK RGLIVRGRHP 190 200 210 220 230 240 VGWCPRHQMP VGAHDTKDDK EPEIGQWTLI YFVDGEGLVY PTATLRPETV PGVTNIWINP 250 260 270 280 290 300 DAEYVVAEFE GRKMVLSKDA AYRLSFQGNV KVIREARGRE FVGRRVLNPV TGEWVPVYEA 310 320 330 340 350 360 KFVDPKVGTG VVMSVPAHAP YDYAALRDMG EVKLIPLIRV EGYGEYPAKE VVERMGIKSQ 370 380 390 400 410 420 TDPALEEATR EVYSAEYTRG VVREDVVDRI APHLPEPARS MVRAVFKLYF AGRPVKEARE 430 440 450 460 470 480 FISKWLAEAG LGGVMYDIMN KPVYCRCGTE IVVKVLEDQW FINYGERGWK QLARQLVEEM 490 500 510 520 530 540 AIIPQEAKAQ FLATIDWLDK RACARTRGLG TPLPWSQGWV IESLSDSTIY MAFYTVIKKI 550 560 570 580 590 600 RALGLRPEQL TEEFWDYVFL GQGSAAEVAK RIGVDPAALE EIRREFDYWY PLDSRNSGKD 610 620 630 640 650 660 LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD KAVALYGPDP 670 680 690 700 710 720 LRATLAIAAE VEQDLDFRDA EARRNSQQLM SIYNLVQRLA QSAVEREETW LDKWLISEVA 730 740 750 760 770 780 HVLERAREAY EKVRLRQAAV ELLYNAEAVF SQYLSMVDKP SKSAVEAAKA WVVALEPIVP 790 800 810 820 830 840 HFAEELWQIL GGEGFAATAP WPKLSPDPAA LLAKRYVDML IEDVKNIPAY KAGAKRVAIY 850 860 870 880 890 900 VNGNYQWLRA AVGKDVKAAI EAGAPPQLAK RLVDFAKSMG EEVRGLVERV EQFDEYAALQ 910 920 930 940 SYKRYVEKAL GVPVDIYKAD DPQAPDLGGK KKAALPLKPG IFIEVG

References

[1]

"Complete sequence of Pyrobaculum calidifontis JCM 11548." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 11548 / VA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000561 Genomic DNA. Translation: ABO08117.1.
RefSeq	YP_001055583.1. NC_009073.1.
3D structure databases
ProteinModelPortal	A3MU00.
ModBase	Search...
Protein-protein interaction databases
STRING	410359.Pcal_0691.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABO08117; ABO08117; Pcal_0691.
GeneID	4908582.
KEGG	pcl:Pcal_0691.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000216620.
KO	K01869.
OMA	LRTFTIP.
ProtClustDB	PRK12300.
Enzyme and pathway databases
BioCyc	PCAL410359:GIX2-710-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_A. Leu_tRNA_synth_A.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR020791. Leu-tRNA-lgase_arc. IPR004493. Leu-tRNA-synth_Ia_arc/euk. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00395. leuS_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_PYRCJ

Accession

Primary (citable) accession number: A3MU00

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	May 29, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program