Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A3MU00 (SYL_PYRCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pcal_0691
OrganismPyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]
Taxonomic identifier410359 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum

Protein attributes

Sequence length946 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 946946Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009404

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif638 – 6425"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3MU00 [UniParc].

Last modified April 3, 2007. Version 1.
Checksum: F0D3BC3EC9CB671B

FASTA946107,481
        10         20         30         40         50         60 
MSELAKFFIE VAEKWQRRWA EAKVFEPSPQ PGRPKFFITA AYPYPNGTIH IGHGRTYLVA 

        70         80         90        100        110        120 
DVMARFRRHL GYNVLFPMAF HYTGTPILTI AEVIAAGDKA VIEEYKEIYG VSDDDIKKMG 

       130        140        150        160        170        180 
DPLYLAQYFH RRSKEAMIKF GLGIDWSREF TTIDPEYQRF IQWQFEKLRK RGLIVRGRHP 

       190        200        210        220        230        240 
VGWCPRHQMP VGAHDTKDDK EPEIGQWTLI YFVDGEGLVY PTATLRPETV PGVTNIWINP 

       250        260        270        280        290        300 
DAEYVVAEFE GRKMVLSKDA AYRLSFQGNV KVIREARGRE FVGRRVLNPV TGEWVPVYEA 

       310        320        330        340        350        360 
KFVDPKVGTG VVMSVPAHAP YDYAALRDMG EVKLIPLIRV EGYGEYPAKE VVERMGIKSQ 

       370        380        390        400        410        420 
TDPALEEATR EVYSAEYTRG VVREDVVDRI APHLPEPARS MVRAVFKLYF AGRPVKEARE 

       430        440        450        460        470        480 
FISKWLAEAG LGGVMYDIMN KPVYCRCGTE IVVKVLEDQW FINYGERGWK QLARQLVEEM 

       490        500        510        520        530        540 
AIIPQEAKAQ FLATIDWLDK RACARTRGLG TPLPWSQGWV IESLSDSTIY MAFYTVIKKI 

       550        560        570        580        590        600 
RALGLRPEQL TEEFWDYVFL GQGSAAEVAK RIGVDPAALE EIRREFDYWY PLDSRNSGKD 

       610        620        630        640        650        660 
LIPNHLTFFI FNHVAIFPRE KWPRQIVANG WVLREGEKMS KSKRNVLPLD KAVALYGPDP 

       670        680        690        700        710        720 
LRATLAIAAE VEQDLDFRDA EARRNSQQLM SIYNLVQRLA QSAVEREETW LDKWLISEVA 

       730        740        750        760        770        780 
HVLERAREAY EKVRLRQAAV ELLYNAEAVF SQYLSMVDKP SKSAVEAAKA WVVALEPIVP 

       790        800        810        820        830        840 
HFAEELWQIL GGEGFAATAP WPKLSPDPAA LLAKRYVDML IEDVKNIPAY KAGAKRVAIY 

       850        860        870        880        890        900 
VNGNYQWLRA AVGKDVKAAI EAGAPPQLAK RLVDFAKSMG EEVRGLVERV EQFDEYAALQ 

       910        920        930        940 
SYKRYVEKAL GVPVDIYKAD DPQAPDLGGK KKAALPLKPG IFIEVG 

« Hide

References

[1]"Complete sequence of Pyrobaculum calidifontis JCM 11548."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 11548 / VA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000561 Genomic DNA. Translation: ABO08117.1.
RefSeqYP_001055583.1. NC_009073.1.

3D structure databases

ProteinModelPortalA3MU00.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING410359.Pcal_0691.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO08117; ABO08117; Pcal_0691.
GeneID4908582.
KEGGpcl:Pcal_0691.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAHAPFDYL.

Enzyme and pathway databases

BioCycPCAL410359:GIX2-710-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PYRCJ
AccessionPrimary (citable) accession number: A3MU00
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries