Reviewed, UniProtKB/Swiss-Prot A3MTU1 (G3P_PYRCJ)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Pcal_0632

Organism

Pyrobaculum calidifontis (strain JCM 11548 / VA1) [Complete proteome] [HAMAP]

Taxonomic identifier

410359 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Glyceraldehyde-3-phosphate dehydrogenase		PRO_0000300976
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	139 – 141	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	140	1	Nucleophile By similarity
	Binding site	110	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	302	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MTU1-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: B8F7652F67DF435A	FASTA	344	37,895
	10 20 30 40 50 60 MIKVGVVGYG TIGKRIADAV ALQDDMRVVG VVKMTPDYEA KIAAARGFPV YTAADRVEKF 70 80 90 100 110 120 KKAGIEVAGT VEDLVKASDV VVDASPEDVG RENKEKYYRQ LDKRYIFQGG EEADVAEVSF 130 140 150 160 170 180 NALANYDEAR GKRYIRVVSC NTTGITRVLS ALLLNGIGIR KARIFIARRG ADPKEHKKGP 190 200 210 220 230 240 INDVVPNPTA VPSHHGPDVQ TVLKDVDIVT MAVAVPVTIM HMHMAYIELD GPRSRDEVLE 250 260 270 280 290 300 AFAKTPRIFL ADVGSGFQSL AQFIEYARDL GRPRGDFPEV AVFRDSVTVR GDELYLMYGV 310 320 330 340 HQESIVVPEN VDAIRAALGV LPKWQSIEKT DKSLKLFTEG KRYA

References

[1]

"Complete sequence of Pyrobaculum calidifontis JCM 11548." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Cozen A.E., Fitz-Gibbon S.T., House C.H., Saltikov C., Lowe T.M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000561 Genomic DNA. Translation: ABO08058.1.
RefSeq	YP_001055524.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4909422.
GenomeReviews	Gene locus Pcal_0632 in contig CP000561_GR.
KEGG	pcl:Pcal_0632.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006436. Glyceraldehyde-3-P_DHase_2_arc. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_PYRCJ

Accession

Primary (citable) accession number: A3MTU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 3, 2007
Last modified:	November 25, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)