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Protein

DNA ligase

Gene

lig

Organism
Pyrobaculum calidifontis (strain JCM 11548 / VA1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei249ATPUniRule annotation1
Active sitei251N6-AMP-lysine intermediateUniRule annotation1
Binding sitei256ATPUniRule annotation1
Binding sitei271ATPUniRule annotation1
Binding sitei301ATPUniRule annotation1
Binding sitei341ATPUniRule annotation1
Binding sitei416ATPUniRule annotation1
Binding sitei422ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Pcal_0039
OrganismiPyrobaculum calidifontis (strain JCM 11548 / VA1)
Taxonomic identifieri410359 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiThermoprotealesThermoproteaceaePyrobaculum
Proteomesi
  • UP000001431 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000498761 – 583DNA ligaseAdd BLAST583

Interactioni

Protein-protein interaction databases

STRINGi410359.Pcal_0039

Structurei

3D structure databases

ProteinModelPortaliA3MS62
SMRiA3MS62
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347 Archaea
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWIKYKRD
OrthoDBiPOG093Z03L0

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

A3MS62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFGELAKVL AAVESTTQRT TMVKLLTSLF KKAKPEEIDK IVYFILGDLR
60 70 80 90 100
PPWEGVELGV AEKLCIRAIS KATGLSAAEL EGWYKKSGDV GEVARRALAA
110 120 130 140 150
GKRPTLLAFA QQKPLEVAEV YDTLLKVAKA AGEGAQDLKV SLLSSLFSRA
160 170 180 190 200
SPEEAKYIAR FVVGKLRLGV ADMTLIEALS EAFGVSKEGL ERAYHVYPDL
210 220 230 240 250
GRLARHVAEG RPLEEIKITP GVPVLPMLAQ RLSSASEILA KLGGTAICEY
260 270 280 290 300
KYDGERAQIH LREGSVKIFS RRLEDITHAY PDVAKAVKEA VKAREAILEG
310 320 330 340 350
EIVAVDPDTG EMLPFQELMH RKRKHDVAEA MEAYPAVLYL FDLLYVDGED
360 370 380 390 400
LTTQPLIYRR LRLSEVVEEG GDVYIAKWGM FDGAEDVDLF FHEAVSLGTE
410 420 430 440 450
GLICKSPTST YEMGARGWNW IKYKRDYRSE MIDTVDLVVV GAFHGRGKRA
460 470 480 490 500
GLYGAFLLAA YDPSTDMFYT VCKVGSGFTD ADLRRMYDML QPLKIPHRHP
510 520 530 540 550
RVVSKMQPDV WFVPQVVIEV IGAEITLSPL HTCCIGAVKP GVGLAIRFPR
560 570 580
FTGRYRTDKR PEDATTTKEL VEMYQRQKKV PTT
Length:583
Mass (Da):64,840
Last modified:April 3, 2007 - v1
Checksum:i3B3B4E318FC2C215
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000561 Genomic DNA Translation: ABO07479.1

Genome annotation databases

EnsemblBacteriaiABO07479; ABO07479; Pcal_0039
KEGGipcl:Pcal_0039

Similar proteinsi

Entry informationi

Entry nameiDNLI_PYRCJ
AccessioniPrimary (citable) accession number: A3MS62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 3, 2007
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health