A3MNG2 (BIOD_BURM7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	BMA10247_2270

Organism

Burkholderia mallei (strain NCTC 10247) [Complete proteome] [HAMAP]

Taxonomic identifier

320389 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000019550
Regions
	Nucleotide binding	118 – 121	4	ATP By similarity
	Nucleotide binding	178 – 179	2	ATP By similarity
Sites
	Metal binding	15	1	Magnesium 1 By similarity
	Metal binding	19	1	Magnesium 2 By similarity
	Metal binding	57	1	Magnesium 2 By similarity
	Metal binding	118	1	Magnesium 2 By similarity
	Binding site	57	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MNG2 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 5E28F2256905A6FB	FASTA	240	24,874
	10 20 30 40 50 60 MSAPLSLFVT GTDTEIGKTF VSAALLHGFA RAGLRAAAMK PVAAGAYERD GAWRNEDADQ 70 80 90 100 110 120 LDAAANVALP AAIRTPFLLK APAAPHIVAA REGVALDIGT IVDAHRRACE MADVIVVEGV 130 140 150 160 170 180 GGVRVPLADT RDTADLAVAL GLPVVLVVGV RLGCISHALL TAEAIAARGL PLAGWVANRI 190 200 210 220 230 240 DPAMPFADDN IDTLRAWLER EHRAPLLGAL AHMSPPSPDA ASHALDVNLL LNALRAAAPR

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 10247.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000548 Genomic DNA. Translation: ABO06105.1.
RefSeq	YP_001081798.1. NC_009080.1.
3D structure databases
ProteinModelPortal	A3MNG2.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MNG2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4892753.
GenomeReviews	Gene locus BMA10247_2270 in contig CP000548_GR.
KEGG	bmn:BMA10247_2270.
PATRIC	19146915. VBIBurMal96640_4493.
TIGR	BMA10247_2270.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	INPGLSH.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	BMAL320389:BMA10247_2270-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BURM7

Accession

Primary (citable) accession number: A3MNG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program