A3MN88 (PUR5_BURM7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	BMA10247_2195

Organism

Burkholderia mallei (strain NCTC 10247) [Complete proteome] [HAMAP]

Taxonomic identifier

320389 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_1000046426

Sequences

	Sequence		Length	Mass (Da)
	A3MN88 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: BB25FAB317BCFFFA	FASTA	351	36,913
	10 20 30 40 50 60 MNPPKSAPDA QGLSYRDAGV DIDAGDALVD KIKPFAKKTL RDGVLGGIGG FGALFEVPKK 70 80 90 100 110 120 YREPVLVSGT DGVGTKLKLA FHLNKHDTVG QDLVAMSVND ILVQGAEPLF FLDYFACGKL 130 140 150 160 170 180 DVETAATVVK GIATGCELAG CALIGGETAE MPGMYPDGEY DLAGFAVGAV EKSKIIDGST 190 200 210 220 230 240 IAEGDVVLGL ASSGIHSNGF SLVRKIIERA NPDLSADFHG RSLADALMAP TRIYVKPLLA 250 260 270 280 290 300 LMEKIAVKGM AHITGGGLVE NIPRVLRDGL TAELDQHAWP LPPLFQWLQQ HGGVADAEMH 310 320 330 340 350 RVFNCGIGMA VIVSAADADD ALRQLADAGE QVWKIGTVRA SREGEAQTVV V

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 10247.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000548 Genomic DNA. Translation: ABO05033.1.
RefSeq	YP_001081724.1. NC_009080.1.
3D structure databases
ProteinModelPortal	A3MN88.
SMR	A3MN88. Positions 13-350.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MN88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4893725.
GenomeReviews	Gene locus BMA10247_2195 in contig CP000548_GR.
KEGG	bmn:BMA10247_2195.
PATRIC	19146765. VBIBurMal96640_4418.
TIGR	BMA10247_2195.
Phylogenomic databases
HOGENOM	HBG531222.
OMA	GIDMIAM.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	BMAL320389:BMA10247_2195-MONOMER.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BURM7

Accession

Primary (citable) accession number: A3MN88

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program