Reviewed, UniProtKB/Swiss-Prot A3MN08 (LSPA_BURM7)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BMA10247_2113

Organism

Burkholderia mallei (strain NCTC 10247) [Complete proteome] [HAMAP]

Taxonomic identifier

320389 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity.
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000038785
Regions
	Transmembrane	10 – 30	21	Potential
	Transmembrane	32 – 52	21	Potential
	Transmembrane	71 – 91	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	135 – 155	21	Potential
Sites
	Active site	117	1	By similarity
	Active site	144	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MN08-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 26B7E87FBD3D2F2C	FASTA	166	18,099
	10 20 30 40 50 60 MAKTLSKSSG GALAPWLGIS LIVILFDQLT KIAVLKTFAY GAMHALTPFF NLTLIYNRGA 70 80 90 100 110 120 AFGFLATAGG WQRWAFTALG IGATLVICYL LKRHGHQRLF SLSLALILGG ALGNVIDRLI 130 140 150 160 YGHVIDFLDF HVGAWHWPAF NLADSAITVG AVLLIYDELR RVRGAR

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000548 Genomic DNA. Translation: ABO07285.1.
RefSeq	YP_001081644.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4893762.
GenomeReviews	Gene locus BMA10247_2113 in contig CP000548_GR.
KEGG	bmn:BMA10247_2113.
TIGR	BMA10247_2113.
Phylogenomic databases
OMA	HLGGWHF.
Family and domain databases
HAMAP	MF_00161. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
ProDom	PD004304. Peptidase_A8. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BURM7

Accession

Primary (citable) accession number: A3MN08

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)