A3MML4 (RNPH_BURM7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BMA10247_1966

Organism

Burkholderia mallei (strain NCTC 10247) [Complete proteome] [HAMAP]

Taxonomic identifier

320389 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Ribonuclease PH HAMAP MF_00564		PRO_1000024786

Sequences

	Sequence		Length	Mass (Da)
	A3MML4 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: F8F805336267EFC0	FASTA	243	26,052
	10 20 30 40 50 60 MTNSSLRPSG RRADQLRDVR ITRHYTKHAE GAVLVEFGDT KVICTASVAE RVPEFLRERG 70 80 90 100 110 120 QGWLTAEYGM LPRATHTRSD REAARGKQTG RTQEIQRLIG RALRAVFDLN ALGPRTLHLD 130 140 150 160 170 180 CDVIQADGGT RTASITGAFV AAHDAVTKLV AAGRIARSPI TDYVAAISVG VFGGTPVLDL 190 200 210 220 230 240 DYDEDSACDT DMNVVMTGAG GFVEVQGTAE GAPFSRTEMN ALLDLAQAGI GELVRLQRAA LEA

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 10247.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000548 Genomic DNA. Translation: ABO05154.1.
RefSeq	YP_001081500.1. NC_009080.1.
3D structure databases
ProteinModelPortal	A3MML4.
SMR	A3MML4. Positions 7-241.
ModBase	Search...
Protein-protein interaction databases
STRING	A3MML4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4894920.
GenomeReviews	Gene locus BMA10247_1966 in contig CP000548_GR.
KEGG	bmn:BMA10247_1966.
PATRIC	19146321. VBIBurMal96640_4199.
TIGR	BMA10247_1966.
Phylogenomic databases
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	BMAL320389:BMA10247_1966-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BURM7

Accession

Primary (citable) accession number: A3MML4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program