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Protein

Succinylglutamate desuccinylase

Gene

astE

Organism
Burkholderia mallei (strain NCTC 10247)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Transforms N(2)-succinylglutamate into succinate and glutamate.UniRule annotationSAAS annotation

Catalytic activityi

N-succinyl-L-glutamate + H2O = succinate + L-glutamate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotationSAAS annotationNote: Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation
  • Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701ZincUniRule annotation
Metal bindingi73 – 731ZincUniRule annotation
Metal bindingi166 – 1661ZincUniRule annotation
Active sitei229 – 2291UniRule annotation

GO - Molecular functioni

  1. hydrolase activity, acting on ester bonds Source: UniProtKB-HAMAP
  2. succinylglutamate desuccinylase activity Source: UniProtKB-EC
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine catabolic process to glutamate Source: UniProtKB-HAMAP
  2. arginine catabolic process to succinate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation

Keywords - Biological processi

Arginine metabolismUniRule annotationSAAS annotation

Keywords - Ligandi

Metal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBMAL320389:GH97-1723-MONOMER.
UniPathwayiUPA00185; UER00283.
UPA00185; UER00283.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinylglutamate desuccinylaseUniRule annotationSAAS annotation (EC:3.5.1.96UniRule annotationSAAS annotation)
Gene namesi
Name:astEUniRule annotationImported
Ordered Locus Names:BMA10247_1731Imported
OrganismiBurkholderia mallei (strain NCTC 10247)Imported
Taxonomic identifieri320389 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000002284: Chromosome I

Interactioni

Protein-protein interaction databases

STRINGi320389.BMA10247_1731.

Structurei

3D structure databases

ProteinModelPortaliA3MLY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2988.
HOGENOMiHOG000280300.
KOiK05526.
OMAiFPNPNVA.
OrthoDBiEOG613070.

Family and domain databases

HAMAPiMF_00767. Arg_catab_AstE.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
TIGRFAMsiTIGR03242. arg_catab_astE. 1 hit.

Sequencei

Sequence statusi: Complete.

A3MLY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSADSGRD AAWLDDFLAL TLAGDAPPAE AGECAARAVR WRWLGDGLLR
60 70 80 90 100
LEPADAAQRM QSVLVSAGVH GDETAPIELL STLVRDIARG ALPLRCRLLV
110 120 130 140 150
ALGNPGAMRA GERYLDDDLN RLFGGRHAQL AASREAPRAA QLEAAAALFF
160 170 180 190 200
STAGRARGAR WHIDMHTAIR TSVFEQFALL PHTGEPPTRT MFEWLGEAQI
210 220 230 240 250
AAVLLHTTKG STFSHFTAQA CGALACTLEL GKVMPFGAND LSRFAPADAA
260 270 280 290 300
VRRLVSGRRD APRGALPRAF TVVDQITKQS DALELFVAND VPNFTPFARG
310 320 330 340
TLLARDGDYR YAVRHEQERI VFPNPSVKPG LRAGLLVIET TRDTHAALA
Length:349
Mass (Da):37,622
Last modified:April 3, 2007 - v1
Checksum:i1D1F32BB7F0A1AF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000548 Genomic DNA. Translation: ABO06240.1.
RefSeqiYP_001081272.1. NC_009080.1.

Genome annotation databases

EnsemblBacteriaiABO06240; ABO06240; BMA10247_1731.
GeneIDi4891618.
KEGGibmn:BMA10247_1731.
PATRICi19145858. VBIBurMal96640_3975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000548 Genomic DNA. Translation: ABO06240.1.
RefSeqiYP_001081272.1. NC_009080.1.

3D structure databases

ProteinModelPortaliA3MLY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi320389.BMA10247_1731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO06240; ABO06240; BMA10247_1731.
GeneIDi4891618.
KEGGibmn:BMA10247_1731.
PATRICi19145858. VBIBurMal96640_3975.

Phylogenomic databases

eggNOGiCOG2988.
HOGENOMiHOG000280300.
KOiK05526.
OMAiFPNPNVA.
OrthoDBiEOG613070.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00283.
UPA00185; UER00283.
BioCyciBMAL320389:GH97-1723-MONOMER.

Family and domain databases

HAMAPiMF_00767. Arg_catab_AstE.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
TIGRFAMsiTIGR03242. arg_catab_astE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. DeShazer D., Woods D.E., Nierman W.C.
    Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 10247Imported.

Entry informationi

Entry nameiA3MLY6_BURM7
AccessioniPrimary (citable) accession number: A3MLY6
Entry historyi
Integrated into UniProtKB/TrEMBL: April 3, 2007
Last sequence update: April 3, 2007
Last modified: March 4, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.