Reviewed, UniProtKB/Swiss-Prot A3MKW5 (ALR_BURM7)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	BMA10247_1350

Organism

Burkholderia mallei (strain NCTC 10247) [Complete proteome] [HAMAP]

Taxonomic identifier

320389 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Alanine racemase HAMAP MF_01201		PRO_1000065974
Sites
	Active site	35	1	Proton acceptor; specific for D-alanine By similarity
	Active site	253	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	35	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MKW5-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: B29D6F1F142DD41E	FASTA	356	38,334
	10 20 30 40 50 60 MPRPISATIH TAALANNLSV VRRHAAQSKV WAIVKANAYG HGLARVFPGL RGTDGFGLLD 70 80 90 100 110 120 LDEAVKLREL GWAGPILLLE GFFRSTDIDV IDRYSLTTAV HNDEQMRMLE TARLSKPVNV 130 140 150 160 170 180 QLKMNSGMNR LGYTPEKYRA AWERARACPG IGQITLMTHF SDADGERGVA EQMATFERGA 190 200 210 220 230 240 QGIAGARSFA NSAAVLWHPS AHFDWVRPGI MLYGASPSGR AADIADRGLK PTMTLASELI 250 260 270 280 290 300 AVQTLAKGQA VGYGSMFVAE DTMRIGVVAC GYADGYPRIA PEGTPVVVDG VRTRIVGRVS 310 320 330 340 350 MDMLTVDLTP VPQAGVGARV ELWGETLPID DVAARCMTVG YELMCAVAPR VPVRAE

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000548 Genomic DNA. Translation: ABO06813.1.
RefSeq	YP_001080901.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4893421.
GenomeReviews	Gene locus BMA10247_1350 in contig CP000548_GR.
KEGG	bmn:BMA10247_1350.
TIGR	BMA10247_1350.
Phylogenomic databases
OMA	TGLKPAM.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_BURM7

Accession

Primary (citable) accession number: A3MKW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)