Reviewed, UniProtKB/Swiss-Prot A3MJU2 (SYFA_BURM7)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BMA10247_0967

Organism

Burkholderia mallei (strain NCTC 10247) [Complete proteome] [HAMAP]

Taxonomic identifier

320389 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000006805
Sites
	Metal binding	258	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3MJU2-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 5343093C696E0F5E	FASTA	337	37,996
	10 20 30 40 50 60 MDLDQIVADA QQSFEGAADI TTLENEKARF LGKSGALTEL LKGLGKLDPE TRKTEGARIN 70 80 90 100 110 120 VAKQQVEAAL NARRQALADA LLNQRLAAEA IDVTLPGRGA GAGSLHPVMR TWERVEQIFR 130 140 150 160 170 180 SIGFDVADGP EIETDWYNFT ALNSPENHPA RSMQDTFYVD GKDADGRPLL LRTHTSPMQV 190 200 210 220 230 240 RYARMNRPPI KVIAPGRTYR VDSDATHSPM FNQVEGLWID ENVSFADLKG AYTDFLKKFF 250 260 270 280 290 300 ERDDILVRFR PSYFPFTEPS AEIDMMFEHG KNAGKWLEIS GSGQVHPTVI RNMGLDPERY 310 320 330 IGFAFGSGLE RLTMLRYGVQ DLRLFFENDL RFLRQFA

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000548 Genomic DNA. Translation: ABO06722.1.
RefSeq	YP_001080528.2.
3D structure databases
SMR	A3MJU2. Positions 82-337.
ModBase	Search...
Genome annotation databases
GeneID	4894419.
GenomeReviews	Gene locus BMA10247_0967 in contig CP000548_GR.
KEGG	bmn:BMA10247_0967.
TIGR	BMA10247_0967.
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. IPR010978. tRNA_bd_arm. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BURM7

Accession

Primary (citable) accession number: A3MJU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)