Reviewed, UniProtKB/Swiss-Prot A3MI87 (ILVC_BURM7)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BMA10247_0398

Organism

Burkholderia mallei (strain NCTC 10247) [Complete proteome] [HAMAP]

Taxonomic identifier

320389 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ketol-acid reductoisomerase		PRO_1000050486
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A3MI87-1 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: 7F06A53243A44DA0	FASTA	338	36,267
	10 20 30 40 50 60 MKVFYDKDAD LSLIKGKQVT IIGYGSQGHA HALNLKDSGV NVTVGLRRGG ASWSKAENAG 70 80 90 100 110 120 LAVKEVAEAV KGADVVMMLL PDEQIAAVYA QEVHANIKEG AALAFAHGFN VHYGQVIPRA 130 140 150 160 170 180 DLDVIMVAPK APGHTVRGTY AQGGGVPHLI AVAQDKSGAA RDIALSYAAA NGGGRAGIIE 190 200 210 220 230 240 TNFREETETD LFGEQAVLCG GTVELIKAGF ETLVEAGYAP EMAYFECLHE LKLIVDLIYE 250 260 270 280 290 300 GGIANMNYSI SNNAEYGEYV TGPRVVTEET KKAMKQCLTD IQTGEYAKSF ILENKAGAPT 310 320 330 LQSRRRLTAE HQIEQVGSKL RAMMPWIAKN KLVDQSKN

References

[1]	DeShazer D., Woods D.E., Nierman W.C. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000548 Genomic DNA. Translation: ABO07112.1.
RefSeq	YP_001079973.1.
3D structure databases
SMR	A3MI87. Positions 1-327.
ModBase	Search...
Genome annotation databases
GeneID	4894061.
GenomeReviews	Gene locus BMA10247_0398 in contig CP000548_GR.
KEGG	bmn:BMA10247_0398.
TIGR	BMA10247_0398.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BURM7

Accession

Primary (citable) accession number: A3MI87

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 3, 2007
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)