A3MAA0 (ILVD_ACIBT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	A1S_3455

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089361
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A3MAA0 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: D9C5512C4CB62BEC	FASTA	609	65,327
	10 20 30 40 50 60 MPDYRSKTST HGRNMAGARG LWRATGMKDE DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AAEIQAAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRDLI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPV VFVSGGPMEA GKVKFRGDEK AIDLVDAMVV AADDSYTDEE 190 200 210 220 230 240 VAEFERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS IVATHANRKK LFLKAGQLIV 250 260 270 280 290 300 ELAKRYYEQN DASILPRSIA TKAAFKNAMT LDIAMGGSTN TVLHLLAAAN EAEVDFTMDD 310 320 330 340 350 360 IDELSRRVPV LSKVAPAKQD VHMEDVHRAG GIMAILGELD RANLLDVSVP TVHEKTLKDA 370 380 390 400 410 420 LDKWDIIRTE DPDVYEFYRS SPGGVPTQVA FSQNRYYSTL DGDREKGVIR NAEHAFSKDG 430 440 450 460 470 480 GLAVLYGNIA LDGCIVKTAG VDESILKFTG SARVFESQDA AVEAILGNEI KAGDVVVIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGLGKDCAL VTDGRFSGGS SGLSIGHVSP EAAEGGAIGL 550 560 570 580 590 600 VEDGDTIEID IPNRTIHLNI DDATLAHRRT VQEAKGWHPK EERKRKVSKA LKVYAMHTTS AAKGAVRIL

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17978 / NCDC KC 755.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000521 Genomic DNA. Translation: ABO13844.2.
RefSeq	YP_001086446.1. NC_009085.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3MAA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4919011.
GenomeReviews	Gene locus A1S_3455 in contig CP000521_GR.
KEGG	acb:A1S_3455.
NMPDR	fig|400667.4.peg.3563.
PATRIC	20723252. VBIAciBau103176_3485.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
ProtClustDB	PRK12448.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ACIBT

Accession

Primary (citable) accession number: A3MAA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	December 14, 2011
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program