A3M9Y0 (MURC_ACIBT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	A1S_3335

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000336808
Regions
	Nucleotide binding	129 – 135	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A3M9Y0 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 4F18E9DBFCAD79ED	FASTA	482	52,882
	10 20 30 40 50 60 MSPTTAANQA KKLIKVPEMR RIKHIHFVGI GGAGMCGIAE VLANQGYKIS GSDIKASKTT 70 80 90 100 110 120 QQLEENGIKV YIGHEAENIK NANVLVVSTA IDPENPEVKA AIEQRIPIVR RAEMLGELMR 130 140 150 160 170 180 YRHGIAVAGT HGKTTTTSLL TTMLAEENLD PTYVIGGLLN STGVNAALGE SRFIVAEADE 190 200 210 220 230 240 SDASFLYLQP MAAIVTNIDA DHMDTYEGSF DKLKDTFVQF LHNLPFYGLA VVCGDDANIR 250 260 270 280 290 300 EILPRVGRPV ITYGFNEDND IRAIDVEQDG MRSHFTVLRK GREPLRLTIN QPGLHNVLNA 310 320 330 340 350 360 LAAIGVATDE GVSDEAISRA LKGFSGVGRR FQVQGEFELG EGNVKLVDDY GHHPKEVEAT 370 380 390 400 410 420 IKAARQSHPD RRLVMLFQPH RYSRTRDCFD DFIEVLSQVD QLLLLEVYPA GEKPIVGADS 430 440 450 460 470 480 RTLARSIRLR GQVEPILIDP VEGNLQNIMQ NVLQPNDLLL TQGAGNVGAI SVELAQHHLY VK

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17978 / NCDC KC 755.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000521 Genomic DNA. Translation: ABO13724.2.
RefSeq	YP_001086326.1. NC_009085.1.
3D structure databases
ProteinModelPortal	A3M9Y0.
SMR	A3M9Y0. Positions 16-476.
ModBase	Search...
Protein-protein interaction databases
STRING	A3M9Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4918130.
GenomeReviews	Gene locus A1S_3335 in contig CP000521_GR.
KEGG	acb:A1S_3335.
NMPDR	fig|400667.4.peg.3435.
PATRIC	20723008. VBIAciBau103176_3363.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
ProtClustDB	PRK00421.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACIBT

Accession

Primary (citable) accession number: A3M9Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	December 14, 2011
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program