Reviewed, UniProtKB/Swiss-Prot A3M7M1 (PROB_ACIBT)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	A1S_2497

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity.
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Glutamate 5-kinase HAMAP MF_00456		PRO_1000125206
Regions
	Domain	285 – 363	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	A3M7M1-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 324324DDF331FEDD	FASTA	377	40,893
	10 20 30 40 50 60 MIEVVDGQRK LSECKRIVVK IGSSLLTANG QGLDLDAISH WAKQIADLHN AGHEIILVSS 70 80 90 100 110 120 GAVAEGMVRM KLASRPTDLP SLQACAAIGQ MGLIHTWSSV LENHSIRTAQ VLLTHDDLAD 130 140 150 160 170 180 RRRYLNSCDA LQNLIDWRVI PVINENDTVS TDEIRFGDND TLAAMVAGQV HADLLIILTD 190 200 210 220 230 240 QQGMFDSDPR HNPDAKLLST VRAMDDVLFE MAGGGGVLGR GGMVTKVRAA RLAAKSGCPT 250 260 270 280 290 300 LIASGESDNV LSRVMAGEML GTLFTTDKDR MTAHQQWLAA HLQTAGRLVI DDGAVEAIKL 310 320 330 340 350 360 KHRSLLPVGV KTVEGHFDRG DVVECVDKQG KRVAVGRVNF SSRSAEIIKG LSSDKVYQVL 370 GEARSLEMIH RDHMAIY

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000521 Genomic DNA. Translation: ABO12915.2.
RefSeq	YP_001085517.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3M7M1.
Genome annotation databases
GeneID	4919515.
GenomeReviews	Gene locus A1S_2497 in contig CP000521_GR.
KEGG	acb:A1S_2497.
NMPDR	fig|400667.4.peg.2602.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_ACIBT

Accession

Primary (citable) accession number: A3M7M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	September 22, 2009
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)