A3M741 (QUEF_ACIBT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	A1S_2313

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm By similarity HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000193206

Sequences

	Sequence		Length	Mass (Da)
	A3M741 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: AA26E8EE8E02D7B1	FASTA	270	30,978
	10 20 30 40 50 60 MSVEQSLLGK ETQYPTSYQP DVLFPIARAQ SREKYAHIEG ITQGKDWWHV FEISWLNAHG 70 80 90 100 110 120 IPQVAIGRIT LPASSPNLIE SKSLKLYFNS LNFTQFDSTQ SFIETVEKDL SAAAGAKVEL 130 140 150 160 170 180 TLFQVDDLEI SKPQGICIDD LMPERLEQHP DATLLKLDES DEEIEVELYS HLLRSNCPVT 190 200 210 220 230 240 GQPDWGTVFI RFKGKKPCYR SILAYIISYR QHNGFHEQCV EQIFADIWQN LQPEKLMVYA 250 260 270 TYTRRGGLDI NPCRVSDLTW MPKPIRLARQ

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17978 / NCDC KC 755.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000521 Genomic DNA. Translation: ABO12735.2.
RefSeq	YP_001085337.1. NC_009085.1.
3D structure databases
ProteinModelPortal	A3M741.
ModBase	Search...
Protein-protein interaction databases
STRING	A3M741.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4919806.
GenomeReviews	Gene locus A1S_2313 in contig CP000521_GR.
KEGG	acb:A1S_2313.
NMPDR	fig|400667.4.peg.2422.
PATRIC	20720920. VBIAciBau103176_2361.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG289139.
ProtClustDB	PRK11792.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_ACIBT

Accession

Primary (citable) accession number: A3M741

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	December 14, 2011
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program