Reviewed, UniProtKB/Swiss-Prot A3M394 (ASPD_ACIBT)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	A1S_0956

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000140084
Sites
	Active site	216	1	By similarity
	Binding site	120	1	NAD; via amide nitrogen By similarity
	Binding site	186	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3M394-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: A2F94A128F512144	FASTA	263	27,954
	10 20 30 40 50 60 MKKLMMIGFG AMAAEVYAHL PQDLQLKWIV VPSRSIEKVQ SQVSSDIQVI SDIEQCDGTP 70 80 90 100 110 120 DYVIEVAGQA AVKEHAQKVL AKGWTIGLIS VGTLADSEFL VQLKQTAEKN DAHLHLLAGA 130 140 150 160 170 180 IAGIDGISAA KEGGLQKVTY KGCKSPKSWK GSYAEQLVDL DHVSEPTVFF TGTAREAAMK 190 200 210 220 230 240 FPANANVAAT IALAGLGMDE TMVELTVDPT INKNKHTIVA EGGFGQMTIE LVGVPLPSNP 250 260 KTSTLAALSV IRACRNSVEA IQI

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000521 Genomic DNA. Translation: ABO11388.2.
RefSeq	YP_001083990.2.
3D structure databases
SMR	A3M394. Positions 3-263.
ModBase	Search...
Protein-protein interaction databases
STRING	A3M394.
Genome annotation databases
GeneID	4917096.
GenomeReviews	Gene locus A1S_0956 in contig CP000521_GR.
KEGG	acb:A1S_0956.
NMPDR	fig|400667.4.peg.982.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1712.
HOGENOM	HBG649642.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPD_ACIBT

Accession

Primary (citable) accession number: A3M394

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	January 19, 2010
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)