A3M2P8 (NUOA_ACIBT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A
NDH-1 subunit A
NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	A1S_0752

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_0000362613
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	63 – 83	21	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A3M2P8 [UniParc]. Last modified April 3, 2007. Version 1. Checksum: E0D568F82F867D31	FASTA	183	19,980
	10 20 30 40 50 60 MSAITPYDWA IIAFVIGVTF LCVFMLTVPL LLGGKSWGRA KQEQFESGVV SAGGARIRLS 70 80 90 100 110 120 AKFYLVAIFF VVFDLEALYL YAWSTSVREV GWLGYTTVVI FVVDLLIALV YAFSVGALSW 130 140 150 160 170 180 APADRRKLAG EKIKVGSPTM NIAEITRFNS IEELVTDPTG QIPAQSSGRV KSKTTPALSS EKE

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17978 / NCDC KC 755.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000521 Genomic DNA. Translation: ABO11192.1.
RefSeq	YP_001083794.1. NC_009085.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3M2P8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4918880.
GenomeReviews	Gene locus A1S_0752 in contig CP000521_GR.
KEGG	acb:A1S_0752.
NMPDR	fig|400667.4.peg.775.
PATRIC	20717697. VBIAciBau103176_0769.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0838.
HOGENOM	HBG605540.
OMA	ALYLYAY.
PhylomeDB	A3M2P8.
ProtClustDB	CLSK830510.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_ACIBT

Accession

Primary (citable) accession number: A3M2P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	April 3, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program