Reviewed, UniProtKB/Swiss-Prot A3M1Z8 (PROA_ACIBT)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	A1S_0489

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000123769

Sequences

	Sequence		Length	Mass (Da)
	A3M1Z8-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 38DCDE995F3359E6	FASTA	421	45,701
	10 20 30 40 50 60 MQDSIEQYMQ KVGQQARDAS RVLTSASTSL KNHALSAIYT ALENNQAAIL AANQIDMEKG 70 80 90 100 110 120 RSNQLDSALL DRLELTPARF KGMLQGLKDV IVLVDPIGEI TDLAYRPTGI QIGKMRVPLG 130 140 150 160 170 180 VVGMIYESRP NVTLEAASLA IKSGNAIILR GGSEALESNK AIAEAVKHGL KVAGLPEHSV 190 200 210 220 230 240 QVIETSDRGA VGHLITMAEY VDVIVPRGGK SLIERVTNEA RIPVIKHLDG NCHVFVEAQA 250 260 270 280 290 300 DLQKALPITL NAKTHRYGVC NAMETLLVDE KIAEVFLPHI AELYAEKQVE LRGCPETRRI 310 320 330 340 350 360 LGSSVKPATE EDWYTEYLGP ILAVKVVSGI DEAIDHINKY GSHHTDAIVT ENYTLARQFL 370 380 390 400 410 420 ARVDSSSVVV NASTRFADGF EYGLGAEIGI STDKIHARGP VGLEGLTSQK WIVLGDGQIR Q

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000521 Genomic DNA. Translation: ABO10942.2.
RefSeq	YP_001083544.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3M1Z8.
Genome annotation databases
GeneID	4918854.
GenomeReviews	Gene locus A1S_0489 in contig CP000521_GR.
KEGG	acb:A1S_0489.
NMPDR	fig|400667.4.peg.489.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACIBT

Accession

Primary (citable) accession number: A3M1Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	October 13, 2009
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)