A3M1A8 (AMPA_ACIBT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	A1S_0227

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000098298
Sites
	Active site	263	1	Potential
	Active site	337	1	Potential
	Metal binding	251	1	Manganese 2 By similarity
	Metal binding	256	1	Manganese 1 By similarity
	Metal binding	256	1	Manganese 2 By similarity
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	333	1	Manganese 1 By similarity
	Metal binding	335	1	Manganese 1 By similarity
	Metal binding	335	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3M1A8 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: E11507C7B3B71401	FASTA	482	52,177
	10 20 30 40 50 60 MKFTTYTTFP EQTSNESLWI LVDSEQLQSN LNTYQINNLE SILTATQFKA NFNETLPLFG 70 80 90 100 110 120 QLSTQPHSQL LGLGKAAELQ AAKLAKLAQT IIKSAQNKFK HIAIDIAALP VEYHYLFALS 130 140 150 160 170 180 LTQAAYGYDE FKSKKNEFVL QQVDLISSQT SLDENQLALV HAVQSGQSYA RDLGNRPGNI 190 200 210 220 230 240 CFPEYLAEQA LALAAEFPDL LKVTVLNEQQ MADLGMYAFL AVSKGSERPG RIVTLEYQAQ 250 260 270 280 290 300 LEQAPVVLVG KGVTFDTGGI SLKPGLGMDE MKFDMCGAAS VLGTIRALCE ARLPIHVVGA 310 320 330 340 350 360 IAAAENMPSG KATRPGDIVT TMSGQTVEIL NTDAEGRLVL CDTLTYIKRF NPAVVIDIAT 370 380 390 400 410 420 LTGACVVALG KVLSGLFSPD DTLAAELQQA GEQSFDRVWR MPVIDDYQEL LDSPFADIAN 430 440 450 460 470 480 IGGPHGGAIT AACFLERFTR DYRWAHLDVA GTAWLSGSAK GATGRPVPLL MQFLANRVST NG

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17978 / NCDC KC 755.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000521 Genomic DNA. Translation: ABO10702.2.
RefSeq	YP_001083304.1. NC_009085.1.
3D structure databases
ProteinModelPortal	A3M1A8.
ModBase	Search...
Protein-protein interaction databases
STRING	A3M1A8.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4918337.
GenomeReviews	Gene locus A1S_0227 in contig CP000521_GR.
KEGG	acb:A1S_0227.
NMPDR	fig|400667.4.peg.241.
PATRIC	20716581. VBIAciBau103176_0235.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
ProtClustDB	PRK00913.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ACIBT

Accession

Primary (citable) accession number: A3M1A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 2, 2008
Last modified:	December 14, 2011
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program