Reviewed, UniProtKB/Swiss-Prot A3M0U3 (RNPH_ACIBT)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	A1S_0042

Organism

Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) [Complete proteome] [HAMAP]

Taxonomic identifier

400667 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP MF_00564		PRO_1000129310

Sequences

	Sequence		Length	Mass (Da)
	A3M0U3-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: EF3216AD4D0B0BE6	FASTA	238	26,296
	10 20 30 40 50 60 MRIDQRALDQ LREVKITRNY TRYAEGSVLV EFGHTKVLCT ASIDNSVPRF LKGQGQGWVT 70 80 90 100 110 120 AEYGMLPRST HSRCDREAAR GKQTGRTQEI QRLIGRSLRA MVDLKKLGEN TITIDCDVIQ 130 140 150 160 170 180 ADGGTRTASI TGAAVALVDA MNVLLAQKKI KQDPLKGLVA AISVGMYQDE VLLDLCYEED 190 200 210 220 230 SNCQTDLNVV MTQAGEFIEI QGTAEDKPFT RAQSNAMLEM AEKGIAELIK KQQEALGW

References

[1]

"New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis." Smith M.G., Gianoulis T.A., Pukatzki S., Mekalanos J.J., Ornston L.N., Gerstein M., Snyder M. Genes Dev. 21:601-614(2007) [PubMed: 17344419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000521 Genomic DNA. Translation: ABO10537.2.
RefSeq	YP_001083139.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3M0U3.
Genome annotation databases
GeneID	4919146.
GenomeReviews	Gene locus A1S_0042 in contig CP000521_GR.
KEGG	acb:A1S_0042.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_ACIBT

Accession

Primary (citable) accession number: A3M0U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 2, 2008
Last modified:	September 1, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)