A3M0C9 (RIFK_PICST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
EC=2.7.1.26

Alternative name(s):
Flavin mononucleotide kinase 1

Gene names

Name:	FMN1
ORF Names:	PICST_50354

Organism

Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome]

Taxonomic identifier

322104 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Debaryomycetaceae › Scheffersomyces › Scheffersomyces stipitis

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme By similarity.
Catalytic activity	ATP + riboflavin = ADP + FMN.
Cofactor	Zinc or magnesium By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence similarities	Belongs to the flavokinase family.

Ontologies

Keywords
Ligand	ATP-binding FMN Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Riboflavin kinase		PRO_0000301849
Sites
	Active site	116	1	Nucleophile By similarity
	Metal binding	39	1	Magnesium or zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3M0C9 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 4D63EA7A0ABCDB2F	FASTA	178	20,327
	10 20 30 40 50 60 MARPDTVIPD KPESPYPIVQ DSVVVSGFGR GSSELGIPTA NIPINDDLNQ LETGIYYGWC 70 80 90 100 110 120 QLKPCTLPDE CKTRTNGREV IYNHGKNLRN DDLKVLPMVM SIGWNPFYHL KEKAAEVHIM 130 140 150 160 170 HKFDDFFYGA QIKFNVLGYI RPELDYTTKE ALIEDINLDI KIALEALDRD AYQTYKDL

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000502 Genomic DNA. Translation: ABN68689.2.
RefSeq	XP_001386718.2. XM_001386681.1.
3D structure databases
ProteinModelPortal	A3M0C9.
ModBase	Search...
Protein-protein interaction databases
STRING	4924.PICST_50354.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4841164.
KEGG	pic:PICST_50354.
Phylogenomic databases
eggNOG	COG0196.
HOGENOM	HOG000260803.
KO	K00861.
OMA	IRPELDY.
OrthoDB	EOG4BCHXC.
Enzyme and pathway databases
UniPathway	UPA00276; UER00406.
Family and domain databases
Gene3D	2.40.30.30. 1 hit.
InterPro	IPR023468. Riboflavin_kinase. IPR015865. Riboflavin_kinase_bac/euk. IPR023465. Riboflavin_kinase_domain. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF01687. Flavokinase. 1 hit. [Graphical view]
SMART	SM00904. Flavokinase. 1 hit. [Graphical view]
SUPFAM	SSF82114. Riboflavin_kinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIFK_PICST

Accession

Primary (citable) accession number: A3M0C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program