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A3LYZ4 (NTE1_PICST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysophospholipase NTE1

EC=3.1.1.5
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene names
Name:NTE1
ORF Names:PICST_33358
OrganismScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis) [Complete proteome]
Taxonomic identifier322104 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces

Protein attributes

Sequence length1546 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium By similarity.

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulation

Inhibited by organophosphorus esters By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the NTE family.

Contains 2 cyclic nucleotide-binding domains.

Contains 1 patatin domain.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentEndoplasmic reticulum
Membrane
   DomainRepeat
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionlysophospholipase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15461546Lysophospholipase NTE1
PRO_0000295329

Regions

Topological domain1 – 4545Cytoplasmic By similarity
Transmembrane46 – 6621Helical; Potential
Topological domain67 – 8115Lumenal By similarity
Transmembrane82 – 10221Helical; Potential
Topological domain103 – 15461444Cytoplasmic By similarity
Domain1239 – 1403165Patatin
Nucleotide binding689 – 820132cNMP 1
Nucleotide binding816 – 965150cNMP 2
Motif1270 – 12745GXSXG
Compositional bias342 – 35514His-rich
Compositional bias495 – 646152Ser-rich

Sites

Active site12721 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3LYZ4 [UniParc].

Last modified July 24, 2007. Version 2.
Checksum: 844221F7DC95189F

FASTA1,546171,904
        10         20         30         40         50         60 
MKDSTEALNS IAFAVDTTLS SILPSSLAPP SAPPATSSFL KSIWYAFWWL WSMVVFKIMN 

        70         80         90        100        110        120 
IILLYIPSKI MNALSINFEI TLNLSSILVA LSAIITVCFL VVRYKYLTGY SKDTSDRKTK 

       130        140        150        160        170        180 
GKVNPSASNI NLKNQSLDYV DQKKGHRRTT NYLDEFLSAI KVFGYLEKSV FHELTKNMTT 

       190        200        210        220        230        240 
QKLSHDEILY LDEKLGFSIV VEGVMQVYTR ITENSNVSGG FDPDDDNELN YEKDDVLIIG 

       250        260        270        280        290        300 
NQRYQLLNEV KSGAPLSSLV SVLDLLKPVD SEDSTSDMLH SFNISDDDNI SKIPEISPIS 

       310        320        330        340        350        360 
LPFQGIHAGA KDDSVPPSPI IRPSKTKQLY PEIVARPKSR PHKEHTGHHL HHVHHSGATI 

       370        380        390        400        410        420 
AIIPYSAFQR VQSKYPKATS HIVTMVLARL YKVTFNTIHD YLGLTKEIME SEVKLNTTSS 

       430        440        450        460        470        480 
VRGANLPGYL FDGLLDKIYG ANGINEASLS RKSDFQRASS VNLNKQKTTL CDAKSSRYVL 

       490        500        510        520        530        540 
LDSRLKSTHP GDLLSSVPLS RRSDYYQTHS HPLSADDPLV RSAFPSSKTL SSLSSPTSNL 

       550        560        570        580        590        600 
KRASSNLKFE NIRDRSFSDD REETEETSLR IAVVESIFKI LGISEKSTAM RNLSSFNSGR 

       610        620        630        640        650        660 
SSVSSSIVGL SNLMSADDKF DTNAARVRFD SYNGFSSTAT SISRSSTPIK FYNTINQNQL 

       670        680        690        700        710        720 
HNHHMGDSVS GINISTLSRQ HQANRNSSPT EFNFANVKSD FAKCLEIKSY GPNTTIVEQG 

       730        740        750        760        770        780 
SFNSGLYYVI DGSLDVLYRP SNHGEPSSNR EDNLKKLYSV KSGGVAGYLS SVVGFRSLVT 

       790        800        810        820        830        840 
IRTSKKRGVI VAHISKSDYS KLMDRYYFLQ LPVATKLKKL LSPQILTIDY ALEWCHIPAG 

       850        860        870        880        890        900 
GVLCSQGDLA NGFHIVLSGR FRVVRNKSDR YQGNTSDDDI LGFSDTSMDC SPSSDINNED 

       910        920        930        940        950        960 
LEVLGEYGHG ESIGEVEVLT ASRRTNSLIA VRDSETARIP RTLFEMLSLS NPSIMVKVSR 

       970        980        990       1000       1010       1020 
IVASKVVYKD VLDQSSRNST LIPSSTASHI SNDYKTITIL PTVSGLPVRE FADKLVSALK 

      1030       1040       1050       1060       1070       1080 
AIGRNVIALD QASTLTHLGR HAFDERLAQL KLSGYFAYLE EEYETIVYIC DTPLKSNWTS 

      1090       1100       1110       1120       1130       1140 
TCISQGDCIL LLADAEDDVV ATGIGDYERL LINLKTMART DLCLLHPEKY VEPGSTSIWL 

      1150       1160       1170       1180       1190       1200 
KNRIWVQGHH HIEMEIIRKK DENSVKKRPN IISELASKIG SKTNPSIKST LEDVRLKAIS 

      1210       1220       1230       1240       1250       1260 
SFVKLNTSFV HSDRYKAVQP HKNDFLRLAR ILSNEAVGLV LGGGGSRGIS HVGIVTALER 

      1270       1280       1290       1300       1310       1320 
HGIPVDLIGG TSIGSLVGGL YAKDYNIVSI YGRAKKFSKR VASLWRSVFD LTYPVTSYIT 

      1330       1340       1350       1360       1370       1380 
GYEFNRGIWK IFGFTEIEDF WIRYFCNTTN ITNSTMDIHE SGYSWRFIRA SMSLAGLLPP 

      1390       1400       1410       1420       1430       1440 
IAFQGCMLLD GGYLDNLPVS EMKKKGAKYI IAVDVGSADD RTPMNYGDTL SGFWVLFNRW 

      1450       1460       1470       1480       1490       1500 
NPFSKHPNVP NMMDIQMRLA YVASVNALEA AKKTNGVIYL RPPIDNYATL DFAKFDEIYH 

      1510       1520       1530       1540 
VGLNYADKLF SSWSKNGKLP AIAGMVDKAK IKSGDDKKVL YRRNSI 

« Hide

References

[1]"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis."
Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M.
Nat. Biotechnol. 25:319-326(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000501 Genomic DNA. Translation: ABN68251.2.
RefSeqXP_001386280.2. XM_001386243.1.

3D structure databases

ProteinModelPortalA3LYZ4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4924.PICST_33358.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4840528.
KEGGpic:PICST_33358.

Phylogenomic databases

eggNOGCOG0664.
HOGENOMHOG000048680.
KOK14676.
OMASPWTQTC.
OrthoDBEOG70W3NS.

Family and domain databases

Gene3D2.60.120.10. 4 hits.
InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 2 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 2 hits.
SSF52151. SSF52151. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNTE1_PICST
AccessionPrimary (citable) accession number: A3LYZ4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: February 19, 2014
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families