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Protein

Lysophospholipase NTE1

Gene

NTE1

Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by organophosphorus esters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1272NucleophilePROSITE-ProRule annotation1
Active sitei1390Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi689 – 820cNMP 1Add BLAST132
Nucleotide bindingi816 – 965cNMP 2Add BLAST150

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase NTE1 (EC:3.1.1.5)
Alternative name(s):
Intracellular phospholipase B
Neuropathy target esterase homolog
Gene namesi
Name:NTE1
ORF Names:PICST_33358
OrganismiScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Taxonomic identifieri322104 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces
Proteomesi
  • UP000002258 Componenti: Chromosome 7

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 45CytoplasmicBy similarityAdd BLAST45
Transmembranei46 – 66HelicalSequence analysisAdd BLAST21
Topological domaini67 – 81LumenalBy similarityAdd BLAST15
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 1546CytoplasmicBy similarityAdd BLAST1444

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002953291 – 1546Lysophospholipase NTE1Add BLAST1546

Interactioni

Protein-protein interaction databases

STRINGi322104.XP_001386280.2.

Structurei

3D structure databases

ProteinModelPortaliA3LYZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1239 – 1403PNPLAPROSITE-ProRule annotationAdd BLAST165

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1243 – 1248GXGXXGPROSITE-ProRule annotation6
Motifi1270 – 1274GXSXGPROSITE-ProRule annotation5
Motifi1390 – 1392DGA/GPROSITE-ProRule annotation3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi342 – 355His-richAdd BLAST14
Compositional biasi495 – 646Ser-richAdd BLAST152

Sequence similaritiesi

Belongs to the NTE family.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 PNPLA (patatin-like phospholipase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2968. Eukaryota.
COG0664. LUCA.
COG1752. LUCA.
HOGENOMiHOG000048680.
InParanoidiA3LYZ4.
KOiK14676.
OMAiSPWTQTC.
OrthoDBiEOG092C0BAH.

Family and domain databases

Gene3Di2.60.120.10. 4 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS51635. PNPLA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3LYZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDSTEALNS IAFAVDTTLS SILPSSLAPP SAPPATSSFL KSIWYAFWWL
60 70 80 90 100
WSMVVFKIMN IILLYIPSKI MNALSINFEI TLNLSSILVA LSAIITVCFL
110 120 130 140 150
VVRYKYLTGY SKDTSDRKTK GKVNPSASNI NLKNQSLDYV DQKKGHRRTT
160 170 180 190 200
NYLDEFLSAI KVFGYLEKSV FHELTKNMTT QKLSHDEILY LDEKLGFSIV
210 220 230 240 250
VEGVMQVYTR ITENSNVSGG FDPDDDNELN YEKDDVLIIG NQRYQLLNEV
260 270 280 290 300
KSGAPLSSLV SVLDLLKPVD SEDSTSDMLH SFNISDDDNI SKIPEISPIS
310 320 330 340 350
LPFQGIHAGA KDDSVPPSPI IRPSKTKQLY PEIVARPKSR PHKEHTGHHL
360 370 380 390 400
HHVHHSGATI AIIPYSAFQR VQSKYPKATS HIVTMVLARL YKVTFNTIHD
410 420 430 440 450
YLGLTKEIME SEVKLNTTSS VRGANLPGYL FDGLLDKIYG ANGINEASLS
460 470 480 490 500
RKSDFQRASS VNLNKQKTTL CDAKSSRYVL LDSRLKSTHP GDLLSSVPLS
510 520 530 540 550
RRSDYYQTHS HPLSADDPLV RSAFPSSKTL SSLSSPTSNL KRASSNLKFE
560 570 580 590 600
NIRDRSFSDD REETEETSLR IAVVESIFKI LGISEKSTAM RNLSSFNSGR
610 620 630 640 650
SSVSSSIVGL SNLMSADDKF DTNAARVRFD SYNGFSSTAT SISRSSTPIK
660 670 680 690 700
FYNTINQNQL HNHHMGDSVS GINISTLSRQ HQANRNSSPT EFNFANVKSD
710 720 730 740 750
FAKCLEIKSY GPNTTIVEQG SFNSGLYYVI DGSLDVLYRP SNHGEPSSNR
760 770 780 790 800
EDNLKKLYSV KSGGVAGYLS SVVGFRSLVT IRTSKKRGVI VAHISKSDYS
810 820 830 840 850
KLMDRYYFLQ LPVATKLKKL LSPQILTIDY ALEWCHIPAG GVLCSQGDLA
860 870 880 890 900
NGFHIVLSGR FRVVRNKSDR YQGNTSDDDI LGFSDTSMDC SPSSDINNED
910 920 930 940 950
LEVLGEYGHG ESIGEVEVLT ASRRTNSLIA VRDSETARIP RTLFEMLSLS
960 970 980 990 1000
NPSIMVKVSR IVASKVVYKD VLDQSSRNST LIPSSTASHI SNDYKTITIL
1010 1020 1030 1040 1050
PTVSGLPVRE FADKLVSALK AIGRNVIALD QASTLTHLGR HAFDERLAQL
1060 1070 1080 1090 1100
KLSGYFAYLE EEYETIVYIC DTPLKSNWTS TCISQGDCIL LLADAEDDVV
1110 1120 1130 1140 1150
ATGIGDYERL LINLKTMART DLCLLHPEKY VEPGSTSIWL KNRIWVQGHH
1160 1170 1180 1190 1200
HIEMEIIRKK DENSVKKRPN IISELASKIG SKTNPSIKST LEDVRLKAIS
1210 1220 1230 1240 1250
SFVKLNTSFV HSDRYKAVQP HKNDFLRLAR ILSNEAVGLV LGGGGSRGIS
1260 1270 1280 1290 1300
HVGIVTALER HGIPVDLIGG TSIGSLVGGL YAKDYNIVSI YGRAKKFSKR
1310 1320 1330 1340 1350
VASLWRSVFD LTYPVTSYIT GYEFNRGIWK IFGFTEIEDF WIRYFCNTTN
1360 1370 1380 1390 1400
ITNSTMDIHE SGYSWRFIRA SMSLAGLLPP IAFQGCMLLD GGYLDNLPVS
1410 1420 1430 1440 1450
EMKKKGAKYI IAVDVGSADD RTPMNYGDTL SGFWVLFNRW NPFSKHPNVP
1460 1470 1480 1490 1500
NMMDIQMRLA YVASVNALEA AKKTNGVIYL RPPIDNYATL DFAKFDEIYH
1510 1520 1530 1540
VGLNYADKLF SSWSKNGKLP AIAGMVDKAK IKSGDDKKVL YRRNSI
Length:1,546
Mass (Da):171,904
Last modified:July 24, 2007 - v2
Checksum:i844221F7DC95189F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000501 Genomic DNA. Translation: ABN68251.2.
RefSeqiXP_001386280.2. XM_001386243.1.

Genome annotation databases

EnsemblFungiiABN68251; ABN68251; PICST_33358.
GeneIDi4840528.
KEGGipic:PICST_33358.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000501 Genomic DNA. Translation: ABN68251.2.
RefSeqiXP_001386280.2. XM_001386243.1.

3D structure databases

ProteinModelPortaliA3LYZ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi322104.XP_001386280.2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiABN68251; ABN68251; PICST_33358.
GeneIDi4840528.
KEGGipic:PICST_33358.

Phylogenomic databases

eggNOGiKOG2968. Eukaryota.
COG0664. LUCA.
COG1752. LUCA.
HOGENOMiHOG000048680.
InParanoidiA3LYZ4.
KOiK14676.
OMAiSPWTQTC.
OrthoDBiEOG092C0BAH.

Family and domain databases

Gene3Di2.60.120.10. 4 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 2 hits.
PS51635. PNPLA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTE1_PICST
AccessioniPrimary (citable) accession number: A3LYZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: November 30, 2016
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.