Reviewed, UniProtKB/Swiss-Prot A3LV34 (ATG15_PICST)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
ORF Names:	PICST_89908

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	Putative lipase ATG15		PRO_0000317971
Regions
	Topological domain	1 – 32	32	Cytoplasmic By similarity
	Transmembrane	33 – 53	21	Signal-anchor for type II membrane protein
	Topological domain	54 – 628	575	Lumenal By similarity
Sites
	Active site	401	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	261	1	N-linked (GlcNAc...) Potential
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Glycosylation	383	1	N-linked (GlcNAc...) Potential
	Glycosylation	518	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A3LV34-1 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 9EF132D20C6DB3E4	FASTA	628	70,605
	10 20 30 40 50 60 MLAAEKRRLL HKTRPVSASE KPGSIYPQRA LYLLVCFSIA TISLGYLHFI GAIDIGRFGI 70 80 90 100 110 120 SSVISDLTKQ DAVSEPFSLH KEQTSSVHTE SDEQTFELKH IFHHGTGPEN YRLHRRLDIT 130 140 150 160 170 180 PSYLAKHSSY FADFSQRLAQ KRDDSSVDSE NLEDIYNLMD WPDVHKHKNP FTIQLPIKKD 190 200 210 220 230 240 HKKGKVVRLK DRHEPGFLDS YLSYALQVKG DPKILNRIAL EWEDEIEIDI PNMKDKDTLV 250 260 270 280 290 300 SLATISSNAY VKFPKDENEK NKSDWIDVGQ WEPDQENVDL NFGWEDIGLR GHVFVSKDNK 310 320 330 340 350 360 TVVIGIKGTS GAGLPGGGSD ETAGNDKDND NLLFSCCCAR VGYMWTTVCN CYEKTYTCNQ 370 380 390 400 410 420 DCLEKELLRE DKYYQAVLDL YRNVSAIYPP ETTNVWVTGH SLGGALASLL GRTYGLPVVA 430 440 450 460 470 480 FEAPGEMLAT KRLHLPQPPG IPKFMENIWH VGNTADPIYM GVCNGASSTC NVAGYAMETA 490 500 510 520 530 540 CHTGYQCVYD VVTDLGWRVN LLNHRIHTVI DDIILAYNDT AQCIEQPPCR DCFNWRFTSH 550 560 570 580 590 600 DDDVPDEPEM PNPLRPKPKP KPSSSTSDGK NNRISSTATT TISPTSRKAD PTSSDISEPS 610 620 ESPKKCLERT WYGWCSKWGY DGDVDDDQ

References

[1]

"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000499 Genomic DNA. Translation: ABN67059.2.
RefSeq	XP_001385088.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4839141.
GenomeReviews	Gene locus ATG15 in contig CP000499_GR.
KEGG	pic:PICST_89908.
Phylogenomic databases
OMA	HIFHHGT.
Enzyme and pathway databases
BRENDA	3.1.1.3. 81766.
Family and domain databases
InterPro	IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_PICST

Accession

Primary (citable) accession number: A3LV34

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)